VEGF在妊娠肝内胆汁淤积症中的作用以及丹参治疗机制的研究医学.docVIP

VEGF在妊娠肝内胆汁淤积症中的作用以及丹参治疗机制的研究医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：VEGF在妊娠肝内胆汁淤积症中的作用以及丹参治疗机制的研究医学 1 1 资料与方法 3 2 结果 4 3 讨论 5 文2：VEGFb医学 7 1材料和方法 8 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 VEGF在妊娠肝内胆汁淤积症中的作用以及丹参治疗机制的研究医学 文1：VEGF在妊娠肝内胆汁淤积症中的作用以及丹参治疗机制的研究医学 Study of the effects of vascular endothelial growth factor and the therapeutic mechanisms of Salvia Miltiorrhiza Bge in intrahepatic cholestasis of pregnancy \　　【Abstract】 Objective To sudy the effects of vascular endothelial growth factor and the therapeutic mechanisms of Salvia Miltiorrhiza Bge in intrahepatic cholestasis of pregnancy(ICP). Methods Twelve patients with ICP and twelve normal pregnancy women take part in this study,their serum is collected before therapy when in hospital. Endothelial cells are isolated from human umbilical vei choosed among the 10 normal pregnancy according to Jaffe’s method, serum of different concentration of ICP groups and normal pregnancy groups is cocultured with HUVECs for different time point respectively. MTT method is applied to measuring the vitality of expression value of vascular endothelial growth factor(VEGF) is detected by enzyme-linked immunosorbent assay (ELISA) . Results Compared with normal pregnancy , the value of MTT in ICP serum groups,the A value is significantly lower,P. The value of soluble VEGF in ICP serum group is lower than that in the nomal pregnancy serum the serum concetration increases, the value of sVEGF decreases in the ICP groups . Compared with ICP group, the value of MTT and sVEGF in the Salvia Miltiorrhiza Bge therapeutic group increases with the concentration increase,8mg/ml of Salvia Miltiorrhiza Bge is the optical concentration. Conclusion Serum of ICP patients impai HUVECs in vitro and decreases the expression of VEGF. The mechanism of serum of ICP patients impai HUVECs maybe by decreasing the expression of VEGF. Salvia Miltiorrhiza Bge could protect HUVECs agait the injury of ICP serum and promotes the expr