复明片对兔视网膜脱离后视网膜色素上皮细胞增殖的影响.docVIP

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复明片对兔视网膜脱离后视网膜色素上皮细胞增殖的影响.doc

复明片对兔视网膜脱离后视网膜色素上皮细胞增殖的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1:复明片对兔视网膜脱离后视网膜色素上皮细胞增殖的影响 1 1 材料与方法 2 2 结果 5 3 讨论 6 文2:复方中药复明片对兔视网膜脱离后视网膜组织形态的影响 8 0 引言 9 1 材料和方法 9 2 结果 10 3 讨论 12 参考文摘引言: 14 原创性声明(模板) 15 文章致谢(模板) 15 正文 复明片对兔视网膜脱离后视网膜色素上皮细胞增殖的影响 文1:复明片对兔视网膜脱离后视网膜色素上皮细胞增殖的影响 〔Abstract〕 Objective To study the effect of Fuming tablet on proliferation of retinal pigment epithelium (RPE) in rabbits eyes with retinal detachment(RD). Method 96 healthy adult coloured rabbits were randomly divided into four groups: blank group(group A), model comparative group (group B), western medicine treating group(group C) and Fuming tablet treating group (group D). The cell suspeio were prepared with retinal tissues gained at 7th, 14th and 21th day after modeling, and the expression of proliferating cell nuclear antigen(PCNA) in retinal neuroseory layer was examined by flow cytometry(FCM). Result The expression of PCNA increased in the four groups after detachment, while it began to decrease after 7th day, and the expression in group D was significant than that in other groups(P). Conclusion Fuming Tablet can inhibit the expression of PCNA by the contact of both retinal neuroseory layer and retinal pigment epithelium layer was recovered by so was prevented. 〔Key words〕 Fuming tablet; retinal pigment epithelium; proliferating cell nuclear antigen; flow cytometry; rabbits; Radix astragali; Rhizome of rehmannia; Poria cocos 视网膜脱离(retinal detachment, RD)是指视网膜色素上皮与神经上皮之间的分离,是严重的致盲眼病,可导致眼球萎缩。在众多致盲因素中占第5位,14岁以下儿童占第4位[1]。视网膜脱离手术后的解剖复位率达95%以上,但术后视功能恢复仍不理想。目前,尚缺乏能应用于临床的有针对性的药物。寻求能改善视网膜脱离手术后增进患者视功能的辅助 治疗 ,是临床上亟待解决的问题。 1 材料与方法 材料 动物 成年健康有色家兔96只,体质量~ kg,雌雄各半,由湖南中医药大学实验动物中心提供。动物合格证号:医动字第20-003号。 药品 复明片:由黄芪、生地、茯苓、车前子、地龙、赤芍、红花、白术等中草药按 现代 制剂制备工艺制成, g/片,选用同一批号药物,批号:040208。由湖南中医药大学第一附属 医院 药剂科提供。使用时去掉糖衣,研成粉末,温开水混合,配制成浓度为10%的混悬液;三磷酸腺苷二钠片(Adenosine Disodium Triphosphate Tablets, ATP):20 mg/片,福建古田药业有限公司生产,批号:050827-1;维生素B1(Vitamin B1,VitB1):10 mg/片,湖北华中药业有限公司生产,批号:;维脑路通:

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