闭合型双循环生物人工肝支持系统治疗犬急性肝功能衰竭实验研究.docVIP

闭合型双循环生物人工肝支持系统治疗犬急性肝功能衰竭实验研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：闭合型双循环生物人工肝支持系统治疗犬急性肝功能衰竭实验研究 1 1 材料与方法 3 2 结果 5 3 讨论 7 文2：新型生物人工肝系统治疗急性肝衰竭犬后猪内源性逆转录病毒传播的实验研究 10 1 材料与方法 11 2 结 果 14 3 讨 论 14 参考文摘引言： 15 原创性声明（模板） 17 文章致谢（模板） 17 正文 闭合型双循环生物人工肝支持系统治疗犬急性肝功能衰竭实验研究 文1：闭合型双循环生物人工肝支持系统治疗犬急性肝功能衰竭实验研究 【Abstract】 Objective To study and evaluate the stability, safety and efficacy of closing bicirculating bioartificial liver support system (CBCBALSS) in treatment of acute hepatic failure in canines. Methods Twenty canine models with acute hepatic failure were set up through endside portocaval shunt with common bile duct traaction and then treated with CBCBALSS (treatment group, n=11) or no hepatocyte CBCBALSS (control group, n=9) for six hou. Serum ammonia, biochemical functio, factor Ⅶ, BCAA/AAA and monoethylglycinexylidide (MEGX) in serum of subjects and deity, viability and number of hepatocytes in cell circulation were detected in treatment group. Results The total volume of hepatocyte solution was 200 ml, with number, deity and viability of hepatocyte for 1×1010, 5×107/ml and 98% respectively. During operation, stable clinical vital sig but traient hypoteion within 30 minutes were seen in 16 canines including two with allergic reaction after 15 minutes (one per group), one death due to upper gastrointestinal hemorrhage and one death because of hemorrhage at puncture spot in CBCBALSS group. Before treatment, serum ammonia, ALT, total/direct bilirubin, factor Ⅶ and BCAA/AAA were 150 mmol/L, 400 U/L, 80/55 mmol/L, 35 g/L, 20% and , respectively. After treatment for six hou, serum ammonia and total/direct bilirubin decreased more significantly in treatment group than those in control group. ALT decreased significantly and showed statistical difference at the sixth hour. But there was no statistical difference in factor Ⅶ, BCAA/AAA and ALP between two groups at all time points. There was statistical difference in MEGX at the fit and se