幽门螺杆菌感染对胃黏膜上皮细胞端粒酶活性的影响及其与细胞凋亡的关系临床医学.docVIP

幽门螺杆菌感染对胃黏膜上皮细胞端粒酶活性的影响及其与细胞凋亡的关系临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：幽门螺杆菌感染对胃黏膜上皮细胞端粒酶活性的影响及其与细胞凋亡的关系临床医学 1 1 材料与方法 3 2 结果 4 3 讨论 5 文2：幽门螺杆菌感染与缺铁性贫血的关系临床医学 7 1 资料与方法 8 2 结果 8 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 11 正文 幽门螺杆菌感染对胃黏膜上皮细胞端粒酶活性的影响及其与细胞凋亡的关系临床医学 文1：幽门螺杆菌感染对胃黏膜上皮细胞端粒酶活性的影响及其与细胞凋亡的关系临床医学 【Abstract】 Objective To investigate the influence of infection on hTERT expression and its relatiohip with cellular apoptosis. Methods was detected by urease test and WarthinStarry method. Terminal deoxynucleotidyl traferasemediated deoxyuridine triphosphate biotin nick end labeling (TUNEL) technique and immunohistochemistry were used to detect the apoptotic cells and the expression of human telomerase catalytic subunit (hTERT) in 64 specime including 16 chronic atrophic gastritis, 15 intestinal metaplasia, 14 gastric dysplasia and 19 human gastric carcinoma. Results (1) The positive rate of hTERT protein expression in chronic atrophic gastritis, intestinal metaplasia, gastric dysplasia and human gastric carcinoma was %, %, % and %, which took on a increasing tendency. The positive rate of hTERT in the cases with infection was significantly higher than that in the cases without infection (% vs %, P). (2)The apoptotic index in the cases with hTERT protein expression and infection was ±, which was significantly higher than that in the cases with hTERT protein expression but without infection (±, P). Conclusio(1) The activity of telomerase may take part in all of the process of gastric carcinogenesis. (2) infection can not only activate telomerase but also increase the apoptosis of gastric epithelioid cells, which increases the itability of gastric epithelioid cells and the risk of gastric cancinogenesis. 【Key words】 Gastric carcinoma; ; Telomerase; Cell apoptosis 端粒酶的激活在肿瘤发生中起关键作用［1］。端粒酶活化与细胞凋亡的关系是目前肿瘤研究的热点之一。探讨Hp感染对胃黏膜上皮细胞端粒酶活性的影响及其与细胞凋亡的关系， 是研究Hp致癌机制的一个重要方向。本研究旨在对胃癌演变过程中Hp感染，端粒酶激活及细胞凋亡之间的关系进行初步探讨。 1 材料与方法 材料 随机抽取我院2000-