丹参对糖尿病肾病大鼠MMP2TIMP1TGFβ1和IVC表达的影响药学.docVIP

丹参对糖尿病肾病大鼠MMP2TIMP1TGFβ1和IVC表达的影响药学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：丹参对糖尿病肾病大鼠MMP2TIMP1TGFβ1和IVC表达的影响药学 1 1 材料 3 2 方法 3 3 结果 5 4 讨论 7 文2：丹参对糖尿病肾病大鼠肾脏水通道蛋白2表达的影响临床医学 9 1 材料 9 2 方法 10 3 结果 12 4 讨论 13 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 丹参对糖尿病肾病大鼠MMP2TIMP1TGFβ1和IVC表达的影响药学 文1：丹参对糖尿病肾病大鼠MMP2TIMP1TGFβ1和IVC表达的影响药学 Abstract：ObjectiveTo study the effect of Salvia miltiorrhiza on the expression of MMP-2,TIMP-1,TGF－β1 and IV-C in Kidney of rats with diabetes and its relatiohip with diabetic nephropathy( DN). MethodsSD rats were randomly divided into normal control group, model group, treatment group and observation group, with 10 rats in each group. The type II diabetic rat model was cotructed by employing high glucose and lipid, and low dose STZ, and the animals were sacrificed at the end of 16th week. Immunohistochemistry was adopted to detect the expression of MMP-2,TIMP-1,TGF－β1 and IV-C in renal tissues, meanwhile, serum glucose, urine protein and renal function were also with the treatment group and observation group, the expression of TGF－β1，TIMP-1 and IV-C significantly decreased(P＜）, but MMP-2 expression increased(P). At the same time, the changes of MMP-2 and IV-C expression were significantly correlated with urine protein expression of TIMP-1,TGF－β1 and IV-C are related to DN,Salvia miltiorrhiza has protective effect on DN through affecting the changes of above mentioned indexes. Key words：Kidney damage with dibetes patients; Extracellular matrix; Salvia miltiorrhiza ; Traforming growing factor; Matrix metallo proteinases/ tissue inhibito of matrix metalloproteinases 糖尿病肾病（diabetic nephropathy,DN）是糖尿病（diabetes mellitus,DM）最常见的微血管并发症，是糖尿病患者主要的死亡原因之一。DN的基本病变是细胞外基质(Extracellular matrix，ECM)在肾小球、肾小管积聚导致肾小球基底膜增厚和系膜基质增多，出现结节型与弥漫型肾小球硬化，前者是DN特异性病理损害。本研究观察丹参对STZ实验性糖尿病肾损害大鼠肾脏金属蛋白酶2（MMP-2）、金属蛋白酶组织抑制因子1（TIMP-1）、转化生长因子1（TGF-β1）和IV型胶原蛋白水平的表达、尿白蛋白排泄率的改变，旨在探讨MMP-2、TIMP-1、TGF-β1和IV-C与DN功能和形态学改变的关系以及丹参的调节效应,为丹参防治DN临床应用与开发提供依据。 1 材料