刺五加多糖对人白血病K562细胞作用的研究.docVIP

刺五加多糖对人白血病K562细胞作用的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：刺五加多糖对人白血病K562细胞作用的研究 1 1 材料和方法 2 2 结果 4 3 讨论 4 文2：金莲花黄酮对人乳腺癌细胞作用的研究 5 1 材料与方法 6 2 结果 8 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 11 正文 刺五加多糖对人白血病K562细胞作用的研究 文1：刺五加多糖对人白血病K562细胞作用的研究 【ABSTRACT】 Objective:To investigate whether the Acanthopanax Senticosus can inhibit proliferation of K562 cells and/or can induce apoptosis of human K562 in :Cells（5×104/mL)were got at midexponential phase and then were planted in 96 well plates for 100μL each and in 50mL culture bottles for were cultured for 48 hou with different concentration of Acanthopanax inhibition rates were measured with CCK8 were detected through following ways:the changes of cell morphology were observed under fluorescence microscope with Hoechst33258 staining,agrose electrophrosis was used to detect the DNA changes and FCM was used to observe sub:Acanthopanax Senticosus can inhibit proliferation of K562 cells cultured with Acanthopanax Senticosus presented classical apoptotic morphology changes such as cell contraction （、 、 、 、 / mL）VS（16%、27%、48%、50%、55%),and apoptotic bodies under fluorescence microscope,and showed DNA fragmernt through agrose addition,the apoptosis were detected by flow cytometic analysis and showed apoptosis apoptosis rate was %.Conclusion:Acanthopanax Senticosus can inhibit proliferation and induce cell apoptosis in K562 in vitro. 【KEY WORDS】 Acanthopanax,K562 Cell,Apoptosis 刺五加在中医药学中的应用已有悠久的 历史 ,近年来的研究证明刺五加和人参具有相似的药理作用和临床疗效,可增加机体的免疫和抗癌作用,且本身几乎无毒性,长期用药不会出现不良反应[1],刺五加主要抗肿瘤的药效部分是皂苷、多糖等[1,2],为探索分离其抗肿瘤活性部位的制剂工艺,并对其抗肿瘤活性部位的确定,曾开展过膜分离其不同药效部位,并就不同药效部位对S180肉瘤抗肿瘤和免疫调节的药效作用比较[3]。本实验根据这一特性，以人白血病细胞K562为研究对象,探讨刺五加多糖对K562细胞增殖抑制和凋亡作用，旨在为刺五加的开发应用提供实验依据。 1 材料和方法 材料和议器 K562细胞购自河北医科大学，经河北北方学院实验中心细胞室传代冻存；RPMI1640培养基购自Gibcol公司；胎牛血清购自天津川页生物工程公司；细胞凋亡Hoechst染色试剂盒为江苏碧云天生物技术研究所产品；CCK8为上海同仁化学公司产品；庆大霉素为郑州羚锐制药有限公司产品；其他均为国产分析纯试剂。仪器选用荧光显微镜(BH2型OLympas,日本)及美国Stat Fax2100型酶标仪等。 刺五加多糖的制备 称取经清洗、烘干、去梗后粉碎的刺五加，置于250mL圆底