脾脏切除对实验性脑损伤大鼠生存率及血液脑组织中IL1β含量的影响临床医学.docVIP

脾脏切除对实验性脑损伤大鼠生存率及血液脑组织中IL1β含量的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：脾脏切除对实验性脑损伤大鼠生存率及血液脑组织中IL1β含量的影响临床医学 2 1 材料 3 2 方法 3 3 统计学处理 5 1 各组大鼠致伤后早期死亡率的观察 6 2 各组大鼠致伤后不同时相点血液中IL1β浓度的测定 6 文2：实验性大鼠脑出血急性期血浆和脑组织中胰岛素样生长因子1的含量医学 8 0 引言 10 1 材料和方法 10 2 结果 11 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 脾脏切除对实验性脑损伤大鼠生存率及血液脑组织中IL1β含量的影响临床医学 文1：脾脏切除对实验性脑损伤大鼠生存率及血液脑组织中IL1β含量的影响临床医学 Abstract： Objective To observe the effect of splenectomy on the mortality and IL1β concentration in blood and its mRNA content in traumatic brain tissue of rats with experimental brain trauma,so as to provide effective clinical management for severe traumatic brain injury Adult male SD rats were divided into 3 groups randomly: sham operation on brain and spleen(group A),experimental brain trauma sham operation on spleen(group B),and experimental brain injury splenectomy(group C).Modified Feeneys method was used to create the animal model of experimental brain within 7 days following the experiment was calculated(nA=23，nB=65，nC=65),and IL-1β concentration in blood at 6,12,24 hou,2 days,and 3 days after experiment was examined by ELISA(nA=nB=nC=6);fluorescent quantitation of IL1β mRNA was also The mortality within 7 days after experiment in group A,B,and C was 0%,%,and % respectively(P，significant difference was observed between groups): at day 2,IL1β concentration in group A,B,and C was ±,±(P),± respectively;at day 3,±,±(P),± respectively;IL1β mRNA content was highly increased at 6 hou after experiment and then decreased in both group B and Splenectomy after experimental brain trauma in rat could effectively reduce its mortality,and could inhibit IL1β expression in some level. Key words:brain trauma;splenectomy;mortality;IL1β;rats 颅脑外伤的致残率和死亡率都很高，其中重型颅脑创伤的死亡率仍高达30%以上[1]。继发性脑水肿是患者死亡的关键原因之一，各种前炎症因子的释放如白细胞介素1β(IL1β)等与继发性脑水肿有着密切关系[2]。脾脏是人体最大的免疫器官，机体在应激状态下能够释放多种炎症介质，在炎症反应中发挥重要作用;但一些研究表明，炎症因子的释放能够加重颅脑损伤的程度[3]。本实验旨在明确实验性大鼠颅脑创伤后行脾脏切除对其死亡率的影响，并