阴虚内热证红斑狼疮外周血单一核细胞BCL2基因家族动态研究临床医学.doc

阴虚内热证红斑狼疮外周血单一核细胞BCL2基因家族动态研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：阴虚内热证红斑狼疮外周血单一核细胞BCL2基因家族动态研究临床医学 1 1 临床资料 3 2 研究方法 3 3 结果 4 4 讨论 5 文2：阴虚内热证红斑狼疮治疗前中后PBMC基因动态研究临床医学 7 1 研究对象 7 2 研究方法 8 3 结果 9 4 讨论 9 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 16 正文 阴虚内热证红斑狼疮外周血单一核细胞BCL2基因家族动态研究临床医学 文1：阴虚内热证红斑狼疮外周血单一核细胞BCL2基因家族动态研究临床医学 Abstract：ObjectiveTo study the dynamic expression of the apoptosis-associated BCL-2 gene family in the peripheral blood mononuclear cells (PBMC) of the heat deficiency patients with lupus erythematosus (SLE) before, during and after treatment with hormone and Ziyin Qingre formula. MethodsBefore, during and after treatment, peripheral blood mononuclear cells of the SLE patients with “Yin” asthenia generating intriic heat were separated,then cDNA microarray chip was used to analyze and compard with a healthy control group,in this part, the data of BCL-2 gene family membe were selected out and BCL-2 family gene were found. The expression of BCL-2 and BAG3 were significantly reduced before, during and after treatment, while the expression of BAG1, BCL6 and BCLA21 was increased after of BCL-2 family gene in PBMC of SLE patients with “Yin” deficiency heat were observed. Apoptosis suppression and promotion gene of BCL-2 family were changed by the hormones and Ziyin Qingre formula treatment which led to reducing apoptosis. BCL6 may be one of the target gene which was affected by the treatment of Chinese medicine. To study the different subtypes apoptosis and apoptosis mechanisms of T and B lymphocyte of SLE with different syndrome pattern before and after treatment is very important for estimation on the role of apoptosis in SLE pathogenesis, diagnosis and treatment. Regulation of apoptotic genes may be one of the mechanisms of Ziyin Qingre formula to achieve Yin and Yang in a relative equilibrium. Key words：Yin asthenia generating intriic heat; Systematic lupus erythematosus (SLE); BCL-2; Apoptosis SLE是多基因相关的自身免疫性系统性疾病，