碱性成纤维细胞生长因子在鼠缺血心肌中表达及其促血管新生作用临床医学.docVIP

碱性成纤维细胞生长因子在鼠缺血心肌中表达及其促血管新生作用临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：碱性成纤维细胞生长因子在鼠缺血心肌中表达及其促血管新生作用临床医学 1 1 材料与方法 3 2 结 果 6 3 讨 论 7 文2：丹参注射液促心肌梗塞后大鼠缺血心肌血管新生作用及对相关生长因子的影响研究 8 1 材料与方法 10 2 结果 12 3 讨论 13 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 碱性成纤维细胞生长因子在鼠缺血心肌中表达及其促血管新生作用临床医学 文1：碱性成纤维细胞生长因子在鼠缺血心肌中表达及其促血管新生作用临床医学 【Abstract】 Objective To investigate the expression of basic fibrolast growth factor (bFGF) cDNA mediated by adenovirus shuttle plasmid in ischemic myocardium and observe the impact on angiogenesis and myocardial infarct area. Methods The anterior descending coronary artery was ligated to make acute myocardial infarction models. 42 health SD rats accepted operation and 30 rats survived. The survival rats were divided into control group (n=15) and treatment group (n=15) randomly. A volume of 100 μl (1 μg/μl) pAdTracebFGF or physiological saline was injected directly into the junctional zone. After 4 weeks, the expression of RFP was detected by fluorescence microscope. The expression of bFGF gene was evaluated by RTPCR and western blot. Myocadial capillary counts were calculated to evaluated the proangiogenc effect. Slices of the LV were stained with triphenyltetrazolium chloride to delineate myocardial infarct area. Results After 4 weeks, all animals with gene therapy can detect the expression of RFP. The expression level of bFGF mRNA was higher in the treatment group (± vs ±, P), the expression of bFGF protein could detected only in the treatment group. Compared to the control group, the capillary deity was higher in the treatment group (± vs ±, P). The myocardial infarction area of the treatment group was smaller than the control group 〔(±)% vs (±)%, P〕. Conclusio bFGF gene mediated by adenovirus shuttle plasmid can be efficiently traferred into rat ischemic heart and then stimulate angiogenesis and minimize myocardial infarct area. 【Key words】 Gene trafection; Myocardial infarction; Angiogenesis 治疗性血管新生通过上调血管生长的细胞因子或受体，从