柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化的影响.docVIP

柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化的影响 1 1 材料与仪器 3 2 方法 3 3 结果 4 4 讨论 5 文2：柴胡皂苷d对乙醇损伤原代培养大鼠肝细胞的保护作用及其机制研究 6 1 器材与方法 7 2 结果 9 3 讨论 10 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化的影响 文1：柴胡皂苷d对酒精性肝纤维化大鼠星形细胞活化的影响 Abstract：ObjectiveTo study the effects of saikosaponin-d (SSd) on the activation of Hepatic Stellate Cells(HSC) and to investigate the main mechanism of SS-d in protection of liver rats were randomly divided into 3 groups：normal group,ethanol-fed group, SSd interventional % distilled alcohol and high fat diet were given orally to the rats of the ethanol-fed group and the SS-d group every day,the rats of SSd group were injected intraperitoneally SSd mg·kg-1 once were sacrificed 12 weeks later. The expression of αsmooth muscle actin (αSMA) was detected by immunohistochemistry method. Pathological change in liver tissue was observed under light microscope by and Van-Gieson staining; the content of liver tissue hydroxyproline(Hyp) and liver histopathology were also examined by with model group，SSd could reduce the expression of αSMA markedly , improve the pathological changes of liver tissue and decrease the production of fibe significantly，decrease the levels of serum alanine traaminase (ALT),aspartate traaminase(AST) and the contents of liver Hyp significantly .Conclusiod can inhibit hepatic fibrosis through inhibiting the expression of αSMA in HSC. Key words：SSd； Liver Fibrosis； Hepatic stellate cell； αSMA 长期过量饮酒可引起酒精性肝病，包括肝脂肪变及炎症损伤，严重者可 发展 为肝纤维化，甚至导致难以逆转的肝硬化。酒精性肝纤维化是酒精性肝病发展到肝硬化的重要阶段，是一可逆过程，如能去除不利因素，消除肝纤维化将成功阻断肝硬化的发生［1，2］。研究表明，肝星形细胞（hepatic stellate cell，HSC）是肝纤维化时肝脏细胞中胶原的主要来源细胞，HSC活化是肝纤维化形成的中心环节［3，4］，本实验用白酒灌胃制备大鼠酒精性肝纤维化模型，观察柴胡皂苷d（SSd）对HSC活化的影响，以探讨中药单体SSd对酒精性肝纤维化预防作用及其机制。 1 材料与仪器 动物 SpragueDawley大鼠，雄性，清洁级，体重（200±21）g，北京实验动物管理中心提供。 试剂 SSd（纯度为98％）：昆明长春花科技有限公司提供，临用前以蒸馏水配置；56度红星二锅头白酒,北京红星酿酒厂生产；ALT，AST，羟脯氨酸(Hyp)检测试剂盒,北京中生生物工程公司；αSMA单抗：购于