雷公藤内酯醇对阿尔茨海默病模型大鼠学习记忆和海马核因子κB表达的影响临床医学.docVIP

雷公藤内酯醇对阿尔茨海默病模型大鼠学习记忆和海马核因子κB表达的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：雷公藤内酯醇对阿尔茨海默病模型大鼠学习记忆和海马核因子κB表达的影响临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：胃癌IκBα与核因子κB表达的研究临床医学 6 1资料与方法 7 12主要试剂 8 13方法 8 14统计学处理 8 22细胞核中NF_κB表达 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 雷公藤内酯醇对阿尔茨海默病模型大鼠学习记忆和海马核因子κB表达的影响临床医学 文1：雷公藤内酯醇对阿尔茨海默病模型大鼠学习记忆和海马核因子κB表达的影响临床医学 【Abstract】 Objective To explore the effect of triptolide (TP) on learning and memory abilities and nuclear factorκB (NFκB) expression of hippocampus in model rats with Alzheimer′s disease (AD). Methods 15 male SD rats were equally divided into control, AD model and TP treated groups. The AD model rats were made with bilateral microinjection of aggregated betaamyloid protein (Aβ)140 into hippocampus in rats and the control rats were injected with normal saline. The TP treated group rats were administered TP intraperitoneally after microinjection of aggregated Aβ140 into hippocampus. Morris water maze test was used for behavioral study. The change of NFκB expression was observed with immunohistochemical staining. Results In place navigation test，the mean escape latencies of the AD model group was obviously increased than that of control group, the mean escape latencies of the TP treated group was obviously decreased compared with the AD model group. In spatial probe test，the frequencies of passing through the platform in AD model group were obviously decreased compared with the control group, the frequencies of passing through the platform of the TP treated group were obviously increased as compared with AD model group. The cell number of the nucleus NFκB positive staining in AD model group was higher than that of control group, the cell number of the nucleus NFκB positive staining in TP treated group was lower than that of AD model group. Conclusio TP can inhibit the activation of hippocampal NFκB in AD model rats, by which TP can improve the learning and memory abilities o