睾酮对TNFα作用于内皮细胞表达HO1的影响临床医学.docVIP

睾酮对TNFα作用于内皮细胞表达HO1的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：睾酮对TNFα作用于内皮细胞表达HO1的影响临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：化疗药物对TRAIL在急性白血病原代细胞表达的影响临床医学 6 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 睾酮对TNFα作用于内皮细胞表达HO1的影响临床医学 文1：睾酮对TNFα作用于内皮细胞表达HO1的影响临床医学 【Abstract】 Objective To investigate the effects of testosterone of different concentratio on the process of tumor necrosis factorα(TNFα)influencing the releasing of hemeoxygenase1 (HO1) in human umbilical vein endothelial cells ECV The effects of testosterone of different concentratio with or without its antagonist Flutamide or estrogen recepto antagonist ICI182, 780 on the senescence of ECV304 induced by TNFαwere studied by ELISA quality kit according to the manufacturer itructio strictly. Results Testosterone at low concentratio (3×10-9, 3×10-8 and 3×10-7 mol/L) had the trend of promoting the releasing of HO1 in ECV304 induced by TNFα, most obviously at the concentration of 3×10-8 mol/L(but P vs TNFαstimulated group), whereas higher concentration (3×10-6 mol/L) had stimulated effect on the senescence induced by TNFα (±, P vs TNFα). The estrogen recepto antagonist ICI182, 170 rather than Flutamide at concentration of 1×10-6mol/L inhibited the protection effect induced by testosterone at concentration of 3×10-8mol/L. Doseand time (with optimal secretion occurring at 24h)dependent effect of TNFαinduced the secretion of HO1. Conclusio Testosterone modulates the senescence of ECV304 induced by TNFαin a doserelated manner. And at physiological concentratio, testosterone has the trend of protecting it, mainly by traforming to estrogen acting on estrogen receptor. 【Key words】 Testosterone; Endothelial cells; Hemeoxygenase1;Tumor necrosis factorα 血红素加氧酶1(HO1)亦称为热休克蛋白32(heatshock protein32，HSP32)，是应激过程中体内出现浓度明显增加的一种应激蛋白，广泛分布在各种组织和细胞中，在体内可催化血红素降解，其主要产物是CO、胆红素和铁，它们是重要的生物效应分子。HO1发挥的细胞、组织保护作用显然与其降解血红素的代谢产物密切相关。HO1的抗氧化作用对动脉粥样硬化(AS)斑块的稳定性也起着重要的作用。研究表明,男性40或50岁后体内睾酮水平明显下降,游