大株红景天对百草枯中毒鼠肺组织NFkβ表达的影响.docVIP

大株红景天对百草枯中毒鼠肺组织NFkβ表达的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大株红景天对百草枯中毒鼠肺组织NFkβ表达的影响 1 1材料及试剂 3 文2：丙泊酚对大鼠缺血再灌注肾NFκBTNFα表达的影响临床医学论文 5 1 资料与方法 5 2 结 果 6 3 讨 论 7 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 11 正文 大株红景天对百草枯中毒鼠肺组织NFkβ表达的影响 文1：大株红景天对百草枯中毒鼠肺组织NFkβ表达的影响 Expression of Nuclear Factor Kappa β and Interleukin-1β in Lung Tissue of Paraquat Poisoned Rats and the Protection of Rosin ZHANG TaoQU Yan*WANG Ye Emergency，Affiliated Yanan，Hospital of Kunming Medical Univeity， Kunming 650051，China Abstract：Objective To investigate the expression of nuclear factor kappa β（NF-kβ）in lung tissue of acute paraquat poisoned rats and the protection of Rosin. Methods 120 male rats were randomly divided into 3 groups：normal control group（C20 rats），paraquat poisoning group（PQ50rats）and Rosin intervention group（Rosin 50rats）.The PQ and Rosin group rats were given paraquat by gastric perfusion（25mg/kg）； The C group rats were given saline by gastric rats in Rosin group were protected by intravenous injectio of penis with Rosin （10mg/kg）15min before exposure of paraquat，once a day until killed. The PQ and C group rats were given saline. Histological changes of lung tissues were observed at different time of 12h、1d、3d 、5d and 7d after poisoned to determin expression of NF-kβ The degree lung injury in PQ group was higher than that in C group. But in Rosin group the degree of lung injury was The content of NF-kβp65 in PQ group was higher than that in C group and that were lower in Rosin NF-kβp65 could play an important role in lung injury of poisoned may inhibit the expression of and NF-kβp65 and alleviate lung injury in acute paraquat poisoned rats。 Keywords：Paraquat；NF-kappaB；Rosin 百草枯（Paraquat，PQ）是目前广泛使用的有机杂环类接触性除草剂，人类中毒后可引起多脏器功能损害，其中以急性肺损伤（Acute Lung Injury，ALI）表现最为突出[1]。近年来对百草枯中毒机制已进行了广泛的研究，但是对于百草枯中毒所致急性肺损伤的预防和治疗，仍未找到行之有效的方法。本实验通过建立急性百草枯中毒大鼠模型，研究大株红景天（Rosin）对急性百草枯中毒大鼠 NF-kβ表达的影响，为临床治疗急性百草枯中毒寻求有效药物依据。 1材料及试剂 动物清洁级SD雄性大鼠120只，体重200～250g，昆明医科大学实验动物中心提供。 材料质量分数为