桑枝总黄酮体外抗炎活性及机制研究.doc

桑枝总黄酮体外抗炎活性及机制研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：桑枝总黄酮体外抗炎活性及机制研究 1 1 材料与仪器 3 2 方法 4 3 结果 6 4 讨论 7 文2：开口箭皂苷抗炎活性的研究 8 1 材料 9 2 方法与结果 10 3 讨论 12 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 桑枝总黄酮体外抗炎活性及机制研究 文1：桑枝总黄酮体外抗炎活性及机制研究 Abstract：ObjectiveTo characterize anti-inflammative effect of total flavones from Morus alba L (TFM) on RAW macrophages stimulated with interferon-γ (IFN-γ) / lipopolysaccharide (LPS). MethodsUsing IFN-γ plus LPS to stimulate RAW macrophages as inflammatory cell model. Griess reaction was used for nitric oxide measurement， MTT assay were used for cell proliferation and viability， RT-PCR was used for assaying mRNA expression and Western blot was used for protein expression. ResultsTFM dose-dependently inhibited both NO production and iNOS expression in IFN-γ+LPS stimulated macrophages without inference on cell viability. The mRNA expression of inflammatory cytokines such as IL-1β and IL-6 was significantly inhibited by TFM treatment. TFM suppressed gene expression of COX-2 while increased HO-1 expression. The protein expression of iNOS and p-ERK was inhibited by TFM. ConclusionOur study suggests that TFM may exert protective effects agait inflammation damage through the mechanism of inhibiting excessive NO， reducing pro-inflammatory mediato such as COX-2，IL-1β，IL-6， and increasing HO-1 expression and enhancing total antioxidant ability. The inhibition of iNOS and COX-2 expression by TFM may be partially mediated through extracellular regulated protein kinases (ERK/MAPK) pathway。 Key words：Inflammation; Nitric oxide; iNOS; Cytokines; Total flavones of Morus alba L.; Mechanism 桑枝(Ramulus Mori)是桑科桑属植物桑Morus alba L.的一年生干燥嫩枝。味微苦、性平，归肝经;具祛风湿、利关节、行水气之功效，适用于风寒湿痹、四肢拘挛、脚气浮肿之病症，临床多用于治疗关节肿痛、手足麻木、瘫痪等多种疾病[1]。桑枝醇提物和石油醚、醋酸乙酯及正丁醇提取物处理动物炎症模型有抗炎作用[2～4]。临床使用经验和现代药理研究均提示桑枝具有较好的抗炎效果，但桑枝抗炎药效的物质基础和作用机制尚未阐明。过量的NO对炎症的发生发展起着关键作用，对其诱导型合酶iNOS的调控有望成为治疗炎症的新策略[5]。致炎因子的刺激将活化巨噬细胞MAPK/ERK/NF-κB信号转导通路，细胞因子大量表达，诱导型合酶iNOS/COX-2经诱导产生大量NO和PGs，炎症