关于不同炮制方法对延胡索中有效成分含量的影响.doc

关于不同炮制方法对延胡索中有效成分含量的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：关于不同炮制方法对延胡索中有效成分含量的影响 1 1 仪器与试药 3 2 方法与结果 3 3 讨论 7 文2：不同炮制方法对何首乌有效成分的影响 8 1 九蒸九晒炮制（古法炮制）对何首乌有效成分的影响 8 2 黑豆汁炮制对何首乌有效成分的影响 9 3 高压清蒸炮制对何首乌有效成分的影响 10 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 关于不同炮制方法对延胡索中有效成分含量的影响 文1：关于不同炮制方法对延胡索中有效成分含量的影响 Abstract：Objective To explore the effect of different processes on the contents of active ingredients in Rhizoma Corydalis. Methods Processes of vinegar-boiling， vinegar-frying and alcohol-frying were used to fresh corydalis， boiled corydalis and purchased corydalis. Then the method of extraction by ultrasound with water and by reflux with methanol were used to extract the sample. The contents of Protopine， Tetrahydropalmatine and Dehydrocorydaline in corydalis was determined by RP-HPLC. Agilent TC-C18 (25 mm× mm， 5 μm) column was used at 35 ℃， with % phosphonic acid (pH with triethylamine) (28∶72) as mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 280 nm. Results In the sample extracted by water， the contents of active ingredients in processed products were higher than in corydalis. And in the sample extracted by methanol， it also has the tendency that the contents of active ingredients in processed products were higher than in corydalis. The content of Dehydrocorydaline in fresh corydalis which was processed by vinegar-boiling， vinegar-frying and alcohol- frying was high. The content of Tetrahydropalmatine in purchased corydalis which was processed by water-boiling at production place was high. Conclusion The method of determining active ingredients in Rhizoma Corydalis was established. It was convenient and the result was accurate. The process technology that fresh corydalis was boiled by water and then processed or fresh corydalis was processed in production place can be used。 Key words：Corydalis；Protopine；Dehydrocorydaline；Tetrahydropalmatine；HPLC；process 延胡索为罂粟科植物延胡索Corydalis yanhusuo ，具有活血、利气、止痛功效，临床用