高分子材料专业聚合物研究方法2d.pptx

;;Most Simple and Useless 2D NMR Spectrum;2D NMR: The Details;;;;;;;;;;;15 ml N?Ndimethylacetamide in 700 ml d-chloroform at 29° C;;;;Basic 2D spectrum;;;;;;Nuclear Spin Interactions;Nuclear Spin Interactions;;;;;t;Heteronuclear Coupled Doublet - On Resonance ;Heteronuclear Coupled Doublet - Off Resonance ;Homonuclear Coupled Doublet - On Resonance ;Homonuclear Coupled Doublet - On Resonance ;Homonuclear Coupled Doublet - On Resonance ;2D J-resolved Spectroscopy ;;;;;;;General Two Dimensional Experiment;;;;General Two Dimensional Experiment;General Two Dimensional Experiment;Simplest Spin System - AX;Spin System - A3X;Spin System - AMX;Spin System - AMX;Spin System - A3X;Heteronuclear Selective Population Transfer;a on resonance;1H NMR spectra of small protein;Same protein 2D-COSY;2D-COSY: NH/CHa expanded region;2D-NOESY;Coupling values to setup experiments;Some 3D sequences;3D-HMQC-COSY;HMQC-NOESY;3D-HMQC-NOESY;2D-NOESY vs 3D-HMQC-NOESY;NOESY-HMQC;NOESY-HMQC and HMQC-NOESY;GE-HMQC-TOCSY;2D and 3D HMQC-TOCSY;3D HCCH-TOCSY;3D-NMR: Puzzle approach;HNCO;HNCO 2D and 3D;HNCA;HCACO;HACA(CO)N;HN(CA)CO and HNCOcomplementary experiments;Example of sequential assignment;HN(CO)(CA);2D HN(CO)(CA);4D-NMR;Magnetization Vector Model;;Detection of Transverse Magnetization --the Free Induction Decay;;Dynamic NMR Spectroscopy;2D NMR;;;Slow Exchange Between Two States;;;;HSQC to Monitor H/D Exchange;? Exposed H exchange rapidly ? complex formation protects from exchange ? measure via 2D NMR ;Protection Factors;;Ensemble of Nuclear Spins ;;Tranverse Relaxation Effects Resonance Linewidth; pose R2 : ns-ps versus ms-ms contributions;;;ps-ns motions;Extracting ns-ps motions;i) Dynamic NMR to measure motions over large range in timescales;EXAMPLE: INEPT;INEPT;Practical Points;INEPT;Refocus chemical shift with “echo”;Refocus chemical shift with “echo”;Refocus chemical shift with “echo”;Refocus chemical shift with “echo”;;EXAMPLE: INEPT;Scalar coupling during “echo