悬饮宁方对SPC（特种医学资料）.docVIP

悬饮宁方对SPC（特种医学资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：悬饮宁方对SPC 1 1 材料与方法 3 2 mg／m1，1 mg／kg，每日腹腔注射1次。 4 2 结果 6 3 讨论 7 文2：SPC在企业中的实际应用 9 1 前言 9 2 SPC简介 9 3 SPC推进过程的典型误区 9 4 有效实施SPC的有效措施 11 5 SPC展望 12 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 悬饮宁方对SPC（特种医学资料） 文1：悬饮宁方对SPC Effects of Xuanyinning Recipe on invasion of SPCA1 cells and pathomorphological changes of peritoneum in mice inoculated with sarcoma 180 ABSTRACT Objective: To observe the effects of Xuanyinning Recipe (XYNR) in inhibiting SPCA1 cellular infiltration and on the pathomorphological changes of peritoneum in mice inoculated with sarcoma 180 (S180). Methods: On the bases of isolated culture of mouse peritoneal mesothelial cells， we adjusted and added the human lung adenocarcinoma cell line SPCA1 into the doublelayer culture medium to observe the number of clones formed. We also took out the peritoneum from the mice administered with three different dosages of XYNR and observed its pathomorphological changes with tramission electron microscope. Results: In the in vitro experiment， the number of clones of SPCA1 in culture medium with XYNR (50 μg/ml) decreased distinctly. In the in vivo experiment， it was observed that， in the peritoneum from the XYNRtreated mice inoculated with S180， the mesothelial cells arranged more and more regularly with the increasing of the dosage of XYNR， while the mesothelial cells in the peritoneum of the mice in the control group necrosed and arranged loosely. Conclusion: XYNR can inhibit the invasion of SPCA1 cells. It also can improve the loose arrangement of the peritoneal mesothelial cells in mice inoculated with S180， so as to inhibit the malignant effusion。 KEY WORDS Xuanyinning Recipe; SPCA1 cells; mesothelial cells; sarcoma 180; mice 研究资料表明，大约50%的癌症转移患者最终发生癌性积液［1］。而癌性积液的出现常常预示着疾病的进展和生活质量的下降，表明疾病进入晚期，生存时间4～6个月［2］。我们在临床及实验中观察到中药悬饮宁方能有效地控制癌性积液生长［3］，为了进一步探讨悬饮宁方抑制癌性积液生长的作用机制，我们进行了悬饮宁方对SPCA1细胞浸润作用的体外实验，并通过电镜观察该方对S180腹水瘤小鼠腹膜组织病理形态的影响，现报告