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- 2022-09-07 发布于湖南
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呼吸道感染分离金黄色葡萄球菌的耐药性分析
[摘要]目的了解临床感染分离的金黄色葡萄球菌的耐药性,为其感染诊治选择合理抗菌药物提供理论参考。方法将2015年3月~2018年5月本院送检临床感染标本,按照《全国临床检验操作规程》标准进行金黄色葡萄球菌分离,接种后于36℃、5%co2环境培养、纯化、鉴定,k-b法判别药敏结果,并采用pcr检测耐药基因。结果840份临床感染标本共分离出金黄色葡萄球菌168株,金黄色葡萄球菌对青霉素g、阿莫西林、氯霉素、头孢噻肟、红霉素、四环素、苯唑西林、庆大霉素、环丙沙星、克林霉素、诺氟沙星、左氧氟沙星、头孢吡肟、万古霉素的耐药率分别为89.29%、83.33%、69.05%、61.90%、53.57%、41.67%、33.33%、28.57%、26.19%、23.81%、22.62%、14.29%、2.38%和0,中介率分别为4.76%、5.95%、9.52%、10.71%、15.48%、20.24%、13.10%、53.57%、42.86%、21.43%、19.05%、11.90%、11.90%和0,敏感率分别为5.95%、10.71%、21.43%、27.38%、30.95%、38.10%、53.57%、17.86%、30.96%、54.76%、58.33%、73.81%、85.71%和100.00%,在抗感染治疗中可优先考虑使用万古霉素等敏感药物。结论临床感染金黄色葡萄球菌对常用抗生素有耐药性,应根据药敏试验结果正确选用有效抗菌药物进行治疗,对减少细菌耐药性的发生有重要意义。
[关键词]金黄色葡萄球菌;耐药基因;耐药性;k-b法
[中图分类号]r725.6[文獻标识码]b[文章编号]1673-9701(2020)02-0139-04
analysisofdrugresistanceofstaphylococcusaureusisolatedfromrespiratorytractinfection
yangbozhangcancan
departmentoflaboratory,xinjiangproductionandconstructioncorpsfourthdivisionhospital,yining835000,china
[abstract]objectivetounderstandthedrugresistanceofstaphylococcusaureusisolatedfromclinicalinfectionandprovidetheoreticalreferenceforreasonableuseofantibioticsindiagnosisandtreatmentofinfection.methodsstaphylococcusaureuswereisolatedaccordingtonationalclinicallaboratoryproceduresfromclinicalinfectionspecimenssubmittedbyourhospitalfrommarch2015tomay2018.afterinoculation,culture,purificationandidentificationat36℃and5%co2wereperformed.thek-bmethodwasappliedtodistinguishsensitivity,andpcrwasusedtodetectresistantgenes.resultsamong168strainsofstaphylococcusaureusisolatedfrom840clinicalinfectionspecimens,resistanceratesofpenicilling,amoxicillin,chloramphenicol,cefotaxime,erythromycin,tetracycline,oxacillin,gentamicin,erythromycin,ciprofloxacin,norfloxacin,levofloxacin,cefepime,vancomycinwere89.29%,83.33%,69.05%,61.90%,53.57%,41.67%,33.33%,28.57%,26.19%,23.81%,22.62%,14.29%,2.38%,and0respectively;intermediatesusceptibilityrateswere4.76%,5.95%,9.52%,10.71%,15.48%,20.24%,13.10%,53.57%,42.86%,21.43%,19.0
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