实时定量PCR检测隐球菌性脑膜炎中TRAIL表达（临床医学范文）.docVIP

实时定量PCR检测隐球菌性脑膜炎中TRAIL表达（临床医学范文） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：实时定量PCR检测隐球菌性脑膜炎中TRAIL表达 1 1 材料和方法 3 2 结果 4 文2：实时定量PCR检测隐球菌性脑膜炎中TRAIL表达 5 1 材料和方法 7 2 结果 8 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 11 正文 实时定量PCR检测隐球菌性脑膜炎中TRAIL表达（临床医学范文） 文1：实时定量PCR检测隐球菌性脑膜炎中TRAIL表达 Abstract： Objective To investigate the method of determining the content of TRAIL mRNA by real-time fluorescent quantitation (RFQ)-PCR, to explore the detection value of expression in peripheral blood mononuclear cells (PBMC) of patients with cryptococcal meningitis, and to analyze the correlation between immune indices of cryptococcal meningitis and TRAIL.Methods By designing specific prime and probes, and with human gene GAPDH as an internal reference, RFQ-PCR was employed to determine the levels of expression of TRAIL mRNA in PBMC from 35 healthy people and 35 patients with cryptococcal meningitis, and a single immunoquantitative diffusion method was adopted to determine the levels of IgG, IgA, IgM, C3, and C4. Results Compared with that of healthy people, the expression of TRAIL mRNA in PBMC of the patients with cryptococcal meningitis was significantly decreased (P0.01). The serum levels of C3 and IgG in the patients with cryptococcal meningitis were significantly decreased (P0.01), and were correlated positively with the change of TRAIL. Conclusion The significantly decreased expression of TRAIL mRNA indicates that TRAIL may be involved in the pathologic development of cryptococcal meningitis, thus providing a new approach for the effective treatment of cryptococcal meningitis. Key words： cryptococcal meningitis; TRAIL; polymerase chain reaction; real-time fluorescent quantitation 隐球菌性脑膜炎是新生隐球菌或其变种侵犯中枢神经系统引起的一种深部真菌感染，临床主要表现为亚急性或慢性脑膜炎、脑膜脑炎，少数可表现为颅内占位性病变［1~3］。免疫功能低下的成人，大剂量糖皮质激素及抗肿瘤化疗药物的使用、肿瘤、AIDS、移植后抗排斥药物的使用等因素，导致隐球菌病的发病率越来越高［4~10]。近年来，人们对此病开展了多方面研究，取得了很大的进展。 TRAIL即肿瘤坏死因子相关的凋亡诱导配体，属于肿瘤坏死因子超家族，1995和1996年分别由Wiley［11］ 和Pitti等［12］分离并鉴定。人类TRAIL基因定位于染色体3q26，编码281个氨基酸，分子量为