绿色木霉对番茄幼苗土壤中有效磷含量的影响.docxVIP

PAGE 8 目录 摘要1 Abstract1 1.前言3 2.材料和方法4 2.1供试材料5 2.2 培养基的制备5 2.3 菌种培养5 2.4仪器设备5 2.5技术路线6 2.6指标测定6 2.6.1生长指标测定6 2.6.2有效磷含量测定7 6.3数据处理7 3.结果与分析7 3.1绿色木霉地上指标的影响7 1.1绿色木霉对株高的影响7 3.1.2绿色木霉对茎粗的影响8 3.1.3绿色木霉对叶宽的影响9 3.2绿色木霉对有效磷含量的影响9 4.结论10 5.讨论10 参考文献 12 绿色木霉对番茄幼苗土壤中有效磷含量的影响 　　摘要：磷是土壤中一种重要的矿质营养元素，参与植物的整个生命活动周期。为探究绿色木霉（Trichoderma viride）对番茄幼苗土壤磷元素的活化作用，在盆栽条件下，优化绿色木霉灌根体系，设置四组处理分别施加1.5 ×107 cfu/mL、1.5×108cfu/mL、1.5×109cfu/mL的绿色木霉，以PD培养基处理作为对照，测定接菌后番茄幼苗的株高、叶宽和茎粗。结果显示，施加1.5×108cfu/mL绿色木霉促生效果明显，茎粗、株高显著增加(P0.05)，分别提高50.35%、45.7%；叶宽增幅18.84%，差异不显著，研究发现1.5×108cfu/mL绿色木霉为最适施加浓度。测定该条件下土壤中有效磷含量，结果显示，施加1.5×108cfu/mL绿色木霉后土壤中有效磷含量显著增加(P0 .05），增幅为104%。表明绿色木霉对番茄幼苗土壤中磷元素具有明显的活化作用，显著提高土壤中有效磷含量。为绿色菌肥的深入研究提供理论支撑。 关键词：绿色木霉；番茄；有效磷； Trichoderma viride promotes the absorption of available phosphorus by tomato 　　Abstract: Phosphorus is an important mineral nutrient element in soil and participates in the whole life cycle of plants. In order to explore the activation effect of Trichoderma viride on soil phosphorus of tomato seedlings, the root irrigation system of Trichoderma viride was optimized under pot cultivation conditions. Four groups of treatments were set up with 1.5×107 CFU /mL, 1.5× 108CFU /mL and 1.5× 109CFU /mL, respectively. The plant height, leaf width and stem diameter of tomato seedlings after inoculation were measured with PD medium as control. The results showed that the growth promoting effect of 1.5× 108CFU /mL Trichoderma virifolia was obvious, stem diameter and plant height were significantly increased (P0.05), increased by 50.35% and 45.7%, respectively; The leaf width increase was 18.84%, and the difference was not significant. The optimal concentration of Trichoderma virifolia was 1.5× 108CFU /mL. The soil available P content was measured under these conditions, and the results showed that the soil available P content was significantly increased (P0.05) by 104% after the application of 1.5× 108CFU /mL Trichoderma virifolia. The results showed that Trichoderma virifolia