CKMT1在中华鳖冬眠期卵巢组织中的定位研究.docx

PAGE 8 PAGE 8 论文题目：CKMT1在中华鳖冬眠期卵巢组织中的定位研究摘 要 　　肌酸激酶（CK）是一种与能量代谢功能有关的关键酶，根据亚基和分布位置的不同，大致可分为四种同工酶，而其中线粒体肌酸激酶（CKMT）主要存在于线粒体。本研究以中华鳖作为研究对象，探寻冬眠期中华鳖卵巢中能量代谢的规律。中华鳖是一类在冬季具有冬眠习性的爬行动物，地位比较特殊，具有重要的学术科研价值，以及较高药用价值和多种营养价值。本实验应用免疫组织化学的方法观察中华鳖冬眠期卵巢组织中的定位，探究处于冬眠和非冬眠中华鳖卵巢组织中的肌酸激酶1（CKMT1）定位的分析，为进一步探讨CKMT1在动物繁殖中的生理作用提供帮助。结果显示，在冬眠组和非冬眠组中的中华鳖冬眠期卵巢组织，加一抗步骤时加入CKMT1的切片均表达阳性，加一抗步骤时加入PBS的切片均不表达。而且不论是冬眠组还是非冬眠组的阳性切片中，质和滤泡细胞均表达。综上所述，中华鳖在冬眠期不同条件下卵巢组织中CKMT1的定位结果显示，CKMT1在冬眠期卵巢中发挥作用。 关键词：中华鳖；免疫组化；肌酸激酶；胍基丙酸；CKMT1 ABSTRACT 　　Creatine kinase (CK) is a key enzyme related to the function of energy metabolism. According to the different subunits and distribution, it can be roughly divided into four isoenzymes, and mitochondrial creatine kinase (CKMT) mainly exists in mitochondria. In this study, the Chinese soft shelled turtle (Trionyx sinensis) was used as the research object to explore the energy metabolism in the ovaries of Chinese soft shelled turtle (Trionyx sinensis) during hibernation. Chinese soft shelled turtle (Trionyx sinensis) is a kind of reptile with hibernation habit in winter. It has a special status and has important academic and scientific research value, as well as high medicinal and nutritional value. In this study, immunohistochemical method was used to observe the localization of creatine kinase 1 (CKMT1) in the ovaries of Chinese soft shelled turtle (Trionyx sinensis) during hibernation, and to explore the localization of creatine kinase 1 (CKMT1) in the ovaries of Chinese soft shelled turtle (Trionyx sinensis) during hibernation and non hibernation. The results showed that the expression of ckmt1 was positive in both hibernating and non hibernating ovarian tissues of Trionyx sinensis. The expression of ckmt1 was negative in the sections with first antibody and PBS. Moreover, both follicular stroma and follicular cells were expressed in the positive sections of both hibernating and non hibernating groups. In conclusion, the localization of CKMT1 in ovarian tissue of Trion