[厦大考研 细胞生物学]线粒体，骨架，癌症，凋亡，细胞核（林炆）.pdfVIP

Structure of mit: Inner mitochondrial membranes which convoluted to form cristae encloses a space called the matrix.the spaces between inner mit membrane and outer membrane is called the intermembrance space. Distribution of enzymes: Outer membrane: Porin:form channels permeable to all molecules of 5000 daltons or less. Inner membrane (Impermeability): Electron-transport chain: Carry out oxidation reactions; ATP synthase: Makes ATP in the matrix; Transport proteins: Allow the passage of metabolites (also Contains a high proportion of the double phospholipid cardiolipin) Intermembrane space: Contains several enzymes which use ATP to phosphorylate other nucleotides. Matrix: Enzymes(related to pyruvate oxidation,TCRcycle,ß oxidation of fats,DNA replication, RNA transcription, Protein translation) (also contains Mit DNA, Ribosomes, etc) 生物氧化产生 ATP 的统计 Chemiosmotic theory When electrons are passed through the respiratory chain, protons are translocated across the inner membrane.(The total proton-motive force across the inner mitochondrial membrane consists of a large force due to the membrane potential and a smaller force due to the H+ concentration gradient. Both forces act to drive H+ into the matrix. )The pH and electrical gradient resulting from this links oxidation to phosphorylation. 1 读万卷书 行万里路 Binding mechanism: The 3 a chains and 3 b chains of F1 form 3 sites with each contains a a chain and a b chain. These sites can go through a loose conformation,a tight conformation and a open conformation in turn. loose conformation : the active site can loosely bind ADP + Pi tight conformation : substrates are tightly bound and ATP is formed open conformation : favors ATP release. H+ gradient drives flagllum The flagellum is