响应面法优化提取绿茶中多酚物质 (1).docVIP

响应面法优化提取绿茶中多酚物质 摘要：以绿茶壮叶为原料,研究水浸提法对鲜茶叶中活性物质提取的影响。根据Box-Benhnken的中心组合设计原理,通过SAS统计软件中的响应面分析法,获得单因素水浸提鲜茶叶中茶多酚的优化工艺:茶水比1:54.9(W/V),温度46.2℃,浸提时间9.6 min,水浸提2次。辅助分析表明,浸提工艺对咖啡碱和氨基酸的提取有显著影响。本试验结果可为水浸提与膜法组合工艺分离并浓缩绿茶叶中的有效成分提供重要的试验依据. 　理采 用三因素三水平的响应 面分析 法 ， 依据 回归分析确定最优提取 工艺条件。结果表 明 ， 其最佳 工艺条件 为： 液料　 比为 ４ ．　 ｌ ， ０ ２ｍＭｇ 超声功率为 ４ ６Ｗ， ７ 　 提取 时间为 １ ．　 ｉ， 用该 工 艺条件 ， 多酚 的提取得 率达 到 １． １％ ， ５ １ｍ ｎ 采 茶 ０ ３２ 通　过 响 应 面 法得 到 一 个 能 较 好 预 测 试 验 结 果 的 模 型 方程 。 　关键词 ： 响应 面法； 多酚 ； 茶 超声波提取法　Abstract: with green tea leaves as raw materials, the effect of water extraction on the extraction of active substances from fresh tea leaves was studied. According to the principle of central combination design of Box-Benhnken, the optimization process of tea polyphenols in fresh tea leaves by single factor water extraction was obtained by the response surface analysis method of SAS statistical software: tea water was compared with 1:54.9 (W/V), temperature 46.2 C, extraction time 9.6 min, and water extraction 2 times. Assistant analysis showed that the extraction process had a significant effect on the extraction of caffeine and amino acids. The results of this study can provide important experimental evidence for the separation and concentration of effective components in green tea by the combination of water extraction and membrane process. The response surface methodology based on three factors and three levels was used to determine the optimal extraction conditions according to regression analysis. The results show that the optimum processing conditions are as follows: the ratio of liquid to material is 4.l, the ultrasonic power of 02mMg is 46W and the extraction time of 7 is 1.i. The extraction yield of polyphenols is up to 1.1%, and 51mn is 032 for tea picking. A model equation which can predict the test results is obtained through the response surface method. Key words: response surface methodology; polyphenols; ultrasonic extraction of tea现状分析： 中国是茶的 \t /item/%E8%8C%B6%E6%96%87%E