能源作物柳枝稷体外花器官逆转鉴定.docx

能源作物柳枝稷体外花器官逆转鉴定 能源作物柳枝稷体外花器官逆转鉴定 摘要： 柳枝稷是一种潜在的生物燃料原料，其体外花器官逆转是一种体外诱导重要的繁殖技术。本研究利用柳枝稷的体外花器官，采用不同生根激素的复合处理，通过逆转诱导及直接生根的方式，实现了柳枝稷体外花器官逆转，并探究了逆转诱导的最佳处理方法。 结果表明，逆转诱导的最佳处理方法为在0.1 mg/L NAA和0.01 mg/L IBA的复合生根激素基础上，添加0.1 mg/L GA3和0.1 g/L PVP，逆转率为61.33%，活性为75.67%。同时，与对照组相比，逆转柳枝稷的株高和新生茎长有显著差异，株高减小了59.25%，新生茎长增加了27.21%。此外，本研究还确定了逆转后生长的最佳培养基成分和光照条件。 因此，该研究提供了一种高效的柳枝稷体外花器官逆转方法，可为柳枝稷育种和优化生长环境提供参考，也为其他能源作物体外培养研究提供了借鉴和参考。 关键词：柳枝稷；体外花器官逆转；生根激素；逆转率；活性 Abstract: Milletia pinnata is a potential biomass feedstock, and its in vitro flower reversal is an important propagation technique for inducing regeneration. In this study, we used the in vitro flower organs of M. pinnata, and employed compound treatments of different rooting hormones to induce flower reversal and direct rooting. We explored the optimal method for inducing flower reversal. Our results showed that the optimal method for inducing flower reversal was to add 0.1 mg/L GA3 and 0.1 g/L PVP to a basal rooting hormone treatment of 0.1 mg/L NAA and 0.01 mg/L IBA, with a reversal rate of 61.33% and an activity of 75.67%. In addition, there were significant differences in plant height and new stem length between the flower reversal group and the control group, with a 59.25% decrease in plant height and a 27.21% increase in new stem length. Furthermore, we identified the optimal culture medium composition and lighting conditions for growth after flower reversal. Therefore, this study provides an efficient method for in vitro flower reversal of M. pinnata, which can serve as a reference for breeding and optimizing the growth environment of M. pinnata, as well as provide a reference for other in vitro studies on biomass feedstock. Keywords: Milletia pinnata; in vitro flower reversal; rooting hormones; reversal rate; activity.