DEHP对新生雄性仔鼠胚胎Leydig cell的毒性作用及机制探讨的开题报告.docxVIP

DEHP对新生雄性仔鼠胚胎Leydig cell的毒性作用及机制探讨的开题报告 【摘要】 本研究拟探讨DEHP对新生雄性仔鼠胚胎Leydig细胞的毒性作用及其机制。采用体外实验模拟DEHP对Leydig细胞的影响，通过MTT法和Annexin V-FITC/PI染色法检测DEHP对Leydig细胞活力和细胞凋亡的影响。同时，通过Western blot和荧光定量PCR技术研究DEHP引起Leydig细胞内雄性素合成和泌泻途径的变化。研究结果将有望揭示DEHP对新生仔鼠Leydig细胞的毒性作用及其机制，为深入了解DEHP的毒性机理提供基础数据，请予以考虑。 【关键词】DEHP；Leydig细胞；毒性作用；机制探讨 【Abstract】 This study aims to explore the toxic effects of DEHP on Leydig cells in newborn male mouse embryos and its mechanism. In vitro experiments were used to simulate the effect of DEHP on Leydig cells, and MTT method and Annexin V-FITC/PI staining method were used to detect the effect of DEHP on Leydig cell viability and apoptosis. At the same time, Western blot and fluorescent quantitative PCR technology were used to study the changes in testosterone synthesis and release pathways in Leydig cells caused by DEHP. The research results are expected to reveal the toxic effects and mechanisms of DEHP on Leydig cells in newborn mouse embryos, providing basic data for a deeper understanding of the toxicity mechanisms of DEHP. 【Key words】DEHP; Leydig cells; Toxicity; Mechanism exploration