中药自拟方剂对小鼠的急性毒性和亚急性毒性实验.docxVIP

延边大学本科论文 学校代码： 学 号： 学校代码： 学 号： 本科毕业设计延 边 大 学 本科毕业设计 本　科　毕　业　论　文 题 目：中药自拟方剂对小鼠的急性毒性和亚急性毒性实验 学生姓名：刘晓童 学 院：农学院 专 业：动物医学 班 级： 指导教师： 二〇二三年六月 摘 要 中药几个世纪以来一直被用于预防、治疗和治愈疾病，中药除了直接用作治疗药物外，也是药理药物研发的重要来源。目前，中药独特的治疗方式已经备受关注。但中药有利也有弊，据相关研究报道中药的不合理使用也会有毒性作用。随着中药的广泛使用，其安全性成为了人们最关心的问题。所以在创新药物研发过程中，新兽药的安全性评价是必要的也是药物非临床安全性评价的重要内容。为评价中药自拟方剂“呼宁散”临床应用的安全性，本试验以清洁级昆明小鼠为试验对象，采用急性毒性和亚急性毒性判断其毒性。结果表明：急性毒性试验中，未能测出中药自拟方剂“呼宁散”的LD50，故采用最大耐受量法测得其对小鼠的最大耐受量为40 g/kg，且中药自拟方剂“呼宁散”药物组小鼠体重在初次给药14 d后与空白组相比未出现显著性差异（P＞0.05），亚急性毒性试验中未见各药物组小鼠饮食、饮水、精神状态和主要组织器官形态结构有异常变化，中药自拟方剂“呼宁散”各剂量组小鼠体重在给药7、14、21、28 d后与空白组相比均未出现显著性差异（P＞0.05）；各剂量组小鼠脏器指数与空白组相比也未出现显著性差异（P＞0.05），通过制作病理切片观察主要脏器显微镜下结构，与空白组相比中药自拟方剂“呼宁散”各剂量组均未观察到有意义的病理学改变。 综上所述，中药自拟方剂“呼宁散”安全无毒，且对小鼠无急性毒性和亚急性毒性，这为临床安全应用提供了有效的依据。 关键词：中药自拟方剂；呼宁散；急性毒性试验；亚急性毒性试验；安全性评价 Abstract Traditional Chinese medicine has been used for centuries to prevent, treat and cure diseases, and in addition to its direct use as a therapeutic drug, it is also an important source of pharmacological drug research and development. At present, the unique treatment of traditional Chinese medicine has attracted much attention. However, traditional Chinese medicine has both advantages and disadvantages. According to relevant studies, irrational use of traditional Chinese medicine can also have toxic effects. With the wide use of traditional Chinese medicine, its safety has become the most concerned issue. Therefore, in the process of innovative drug research and development, the safety evaluation of new veterinary drugs is not only necessary but also an important part of the non-clinical safety evaluation of drugs. In order to evaluate the safety of clinical application of Huning SAN, clean grade Kunming mice were taken as test subjects in this experiment. Acute toxicity and subacute toxicity were used to determine its toxicity. The results show that: In the acute toxicity test, the LD50 of the self-made prescription of traditional Chinese medicine Huning SAN could not be measur