土壤修复过程中微生物数量、酶活性与石油降解率的关系.docxVIP

石油学报(石油加工) 2014年2月 ACTA PETROLEI SINICA(PETROLEUM PROCESSING SECTION) 第 3 0 卷 第 1 期 文章编号： 1001-8719(2014)01-0094-06 土壤修复过程中微生物数量、酶活性与 石油降解率的关系 张秀霞，武海杰，白雪晶，郭云霞，张守娟 (中国石油大学环境与安全工程系，山东青岛266580) 摘要： 在优化降解条件下投加高效石油降解菌，在恒温恒湿的培养箱中，用花盆对石油污染土壤进行28d 模 拟 原 位修复。考察修复过程中微生物(细菌、真菌和放线菌)数量、土壤酶活性以及石油降解率的变化，用 SPSS18.0 对 数据进行统计分析，研究微生物数量、土壤酶活性与石油降解率的相关性。结果表明，细菌数量和微生物总数、 脲酶、过氧化氢酶和多酚氧化酶活性与石油降解率相关性非常显著，相关性系数分别为0.989、0.977、0.994、 0.964和0.988;蛋白酶和脱氢酶活性与石油降解率相关性显著，相关性系数分别为0.944和0.934;真菌数量和放 线菌数量与石油降解率相关性不显著。 关 键 词：石油；高效降解菌；微生物数量；酶活性 中图分类号： X53 文献标识码： A doi:10.3969/j.issn.1001-8719.2014.01.015 Relationship Between Microorganism Population Quantity, Soil Enzyme Activity and Oil Degradation Rate in Soil Bioremediation ZHANG Xiuxia, WU Haijie, BAI Xuejing,GUO Yunxia, ZHANG Shoujuan (Department of Environmental and Safety Engineering,China University of Petroleum,Qingdao 266580,China) Abstract: Under optimized degradation conditions, dosing highly efficient petroleum-degrading bacteria to oil contaminated soil, microorganism in-situ remediation in constant temperature and humidity incubator was simulated for 28 d in flowerpot. Through changes of microorganism (bacteria, fungi and actinomycetes) population quantity, soil enzyme activity and oil degradation rate in remediation, the data were statistically analyzed with SPSS18.0 to study the correlations between microorganism population quantity, soil enzyme activity and oil degradation rate. Results showed that the correlations between bacteria quantity and total number of microorganism, urease, catalase and polyphenol oxidase activity and oil degradation rate were very significant with the correlation coefficient of 0.989,0.977,0.994,0.964 and 0.988,respectively. The correlations between protease, dehydrogenase and oil degradation rate were significant with the correlation coefficient of 0.944 and 0.934, respectively. The correlations between fungi qua