庆大霉素对C57BL6J成年小鼠听力损害剂量模式及带状突触可塑性的研究的中期报告.docxVIP

庆大霉素对C57BL6J成年小鼠听力损害剂量模式及带状突触可塑性的研究的中期报告 摘要： 庆大霉素是一种广泛用于临床的抗生素，但在一些情况下会对听力产生不良影响。本研究旨在探讨庆大霉素对C57BL/6J小鼠听力损伤的剂量效应关系以及对带状突触可塑性的影响。我们将小鼠随机分成四组，分别注射庆大霉素1天、3天和5天，以及对照组。每组小鼠均接受听阈测试，记录其听阈值在注射前、注射后以及随后两周内的变化。同时，我们还使用电生理技术记录小鼠上行听皮质的带状突触可塑性，以探究庆大霉素与听力信号传递之间的关系。初步结果表明，庆大霉素对小鼠听力的剂量效应具有明显的阈值性，即在一定剂量范围内会对听力产生明显损伤，但以上下调整剂量均无效果；在小剂量下，庆大霉素对小鼠的带状突触可塑性存在影响，而在大剂量下影响更为明显。我们将继续深入分析数据，以探究此种药物对人类听力健康的潜在影响。 Abstract: Gentamicin is a commonly used antibiotic clinically, but it can have adverse effects on hearing in some cases. The aim of this study was to investigate the dose-effect relationship of gentamicin-induced hearing impairment in C57BL/6J mice and its effect on ribbon synapse plasticity. Mice were randomly divided into four groups and injected with gentamicin for 1, 3, and 5 days respectively, as well as a control group. Each group of mice underwent tone threshold testing, and changes in their threshold values before and after injection and during the following two weeks were recorded. At the same time, we also used electrophysiological techniques to record ribbon synapse plasticity in the mouse ascending auditory cortex to explore the relationship between gentamicin and auditory signal transmission. Preliminary results showed that the dose-effect relationship of gentamicin on mouse hearing had a clear threshold effect, i.e. it caused significant damage to hearing within a certain dose range, but adjusting the dose up or down had no effect. At low doses, gentamicin had an effect on ribbon synapse plasticity in mice, while at high doses the effect was more pronounced. We will continue to analyze the data in depth to explore the potential impact of this drug on human hearing health.