桂皮醛的肠道吸收机制.docxVIP

PAGE 7 中文摘要 　　目的：建立并验证Caco-2细胞模型，模拟人体的肠道环境，以探讨桂皮醛的肠道吸收机制。方法：建立Caco-2 细胞模型，观察细胞形态，测量上皮细胞跨膜电阻值（TEER）来衡量Caco-2细胞单层的紧密性。利用CCK8法检测桂皮醛对Caco-2细胞的毒性以筛选出安全的给药浓度进行实验。通过研究时间、浓度、温度对Caco-2细胞摄取桂皮醛的影响以及桂皮醛的双向转运来研究桂皮醛的转运机制。结果：培养的 Caco-2 细胞生长均匀、致密，边界清晰， Caco-2 细胞单层模型建立成功，桂皮醛作用于Caco-2细胞的安全浓度为0~35 μg/ml，可用于桂皮醛的转运试验。通过比较不同浓度的桂皮醛溶液对Caco-2细胞摄取的影响发现药物的转运量随浓度的增加而基本呈线性变化。比较不同温度对Caco-2细胞摄取的影响发现桂皮醛溶液的Papp值并无显著性差异，表明桂皮醛的转运并不依赖温度来提供能量。桂皮醛的双向转运研究进一步表明桂皮醛转运过程主要通过被动扩散方式转运。结论：桂皮醛的转运过程主要是通过被动扩散的方式进行转运。 关键词：桂皮醛；Caco-2细胞；机制；摄取转运 ABSTRACT 　　Objective: By establishing and verifying the Caco-2 cell model to simulate the intestinal environment of the human body to discuss the intestinal absorption mechanism of the cinnamaldehyde. Methods: Caco-2 cell model was established and the cell morphology was observed, and the epithelial cell transmembrane resistance value was measured to measure the tightness of the single layer of Caco-2 cells. CCK8 method was used to detect the toxicity of cinnamaldehyde to Caco-2 cells in order to screen the safe concentration of cinnamaldehyde. The transport mechanism of cinnamic aldehyde was studied by studying the effects of three different factors, namely time, concentration and temperature on the uptake of cinnamic aldehyde by Caco-2 cells and the two-way transport of cinnamic aldehyde. Results: The cultured Caco-2 cells grew evenly and compactly with clear boundary. The monolayer model of Caco-2 cells was successfully established. The safe concentration of cinnamaldehyde acting on Caco-2 cells was 0~35 μg/ml, which could be used for the transport test of cinnamaldehyde. By comparing the effects of cinnamaldehyde solution with different concentrations on the uptake of Caco-2 cells, it was found that the drug transport quantity changed linearly with the increase of concentration. By comparing the effects of different temperatures on the uptake of Caco2 cells, it was found that there was no significant difference in Papp value of cinnamaldehyde solution, which indicate