一种具有大斯托克斯位移和高光学稳定性的线粒体靶向近红外染料及其细胞成像应用（英文）.docxVIP

一种具有大斯托克斯位移和高光学稳定性的线粒体 靶向近红外染料及其细胞成像应用(英文) A mitochondria-targeting near-infrared dye with large Stokes shift and high optical stability for cellular imaging applications Abstract Mitochondrial imaging is of great significance for studying mitochondrial function and for investigating diseases related to dysfunction in these organelles. Fluorescent dyes have been widely used for imaging mitochondria, however, there are challenges associated with achieving high optical stability and sufficient sensitivity for visualization. In this study, we developed a mitochondria-targeting near-infrared dye with a large Stokes shift and high optical stability for cellular imaging applications. The dye demonstrated excellent mitochondria-targeting ability and low cytotoxicity. Additionally, it displayed a large Stokes shift, which reduces spectral overlap and enables high signal-to-background ratios. This dye provides a powerful tool for mitochondrial imaging and has the potential to advance our understanding of mitochondrial biology and disease. Introduction Mitochondria are organelles that exist within eukaryotic cells, which play a crucial role in energy metabolism, programmed cell death and other cellular processes. Therefore, mitochondrial imaging has significant clinical and research applications, such as in diseases such as cancer, cardiovascular disease and neurological disorders. Fluorescent imaging is commonly used for mitochondrial imaging, but it remains challenging to achieve high optical stability and sufficient sensitivity. This is due to low fluorescence quantum yields of dyes, spectral overlap, and issues with photobleaching, phototoxicity and poor targeting efficiency. In order to overcome these challenges, we have developed a mitochondria-targeting near-infrared dye with a large Stokes shift and high optical stability, which has promising indications for use in cellular im