微生物学史微生物学史 (55).pptxVIP

Microbial Identification After learning this section, you should be able tOutline the methods of identification of the microorganisms; IdentificationClassical methods of microbialclassification and identificatioMordern methods of microbialclassification and identification Classical methods :MorphologicalPhysiologicalBiochemicalEcological Morphological Characteristics Physiological andMetabolic Characteristics Second, The modern methods of classification and identificationThe modern methods of classification and identification were mostly based on molecular [m lekj l (r)]characteristics Nucleic Acid Base Composition: G + C contentIt is taxonomically useful because variation within a genus is usually less than 10% but variation between genera is quite variable ranging from 25% to 80%. Nucleic Acid HybridizationIt determines the degree of sequence homology. Nucleic Acid SequencingThe small subunit rRNAs are almost ideal for studies of microbial evolution, relatedness, and genus identification because they play the same role in all microorganisms. The phylogenetic tree of some microorganisms Complete bacterial genomes A Word listidentification 鉴定morphological 形态学的physiological 生理学的biochemical 生物化学的ecological 生态学的G+C content GC含量hybridization 杂交phylogenetic tree 系统发育树genome 基因组