分子生物学：GH 基因操作.pptVIP

三、Recombinant DNA TechnologyDNA重组技术 Isolation of target gene Selection and construction of vectors Ligation of target DNA and vector Transformation of target gene into receptor cell Screening for recombinant plasmids Expressing a cloned gene　 Process of cloning基因克隆研究程序 　 Process of DNA cloning 　 1. Isolation of target gene Chemical synthesis：only for simple polypeptide chain whose primary structure is clear. Obtaining from genomic DNA library Obtaining from cDNA library polymerase chain reaction (PCR) 　 A few commonly used vectors： plasmid phage cosmid yeast artificial chromosome (YAC) 2