分子生物学：RNA加工与核糖核蛋白复合体.pptVIP

Function of poly(A) tail Increased mRNA stability Increased translational efficiency Splicing of last intron 剪 接 The process of cutting the pre-mRNA to remove the introns and joining together of the exons is called splicing. It takes place in the nucleus before the mature mRNA can be exported to the cytoplasm. Introns: non-coding sequences Exons: coding sequences RNA splicing: removal of introns and joining of exons Splicing mechanism must be precise精确的 to maintain open reading frame保持可读框 Catalyzed by spliceosome剪接体 (RNA + protein) pre-mRNA剪接的生化步骤 Step 1: a cut is made at the 5′splice site,