分子生物学：Section I 基因文库与筛选.pptVIP

Transfer to nitrocellulose硝化纤维 or nylon membrane Denature DNA(NaOH) Bake烤 onto membrane Probe with 32p-labled DNA complementary to gene of interest Expose to film Select positive阳性 from master plate Keep master plate原始平板 Screening by plaque hybridization 用噬菌斑杂交筛选 Identify the protein product of an interested gene Protein activity蛋白质活性 Western blotting using a specific antibody专门的抗体 I3-3 Expression screening表达筛选 Expression screening (1) If the inserts are cloned into an expression sites, it may be expressed. Therefore, we can screen for the expressed proteins. However, this screeni