湖北襄阳地区规模化牛场产气荚膜梭菌的分离和鉴定.docxVIP

湖北襄阳地区规模化牛场产气荚膜梭菌的 分离和鉴定 作者：郑晓丽，窦贤明，胡道俊，等 来源：《湖北农业科学》2011年第23期 郑晓丽1,窦贤明1,胡道俊2,肖 勇1,赵翠荣1,倪学勤3 (1.湖北省襄阳市农业科学院，湖北襄阳4 4 1 0 5 7；2.湖北省安格斯种牛场，湖 北襄阳 4 4 1 1 0 6； 3.四川农业大学动物医学院动物微生态研究中心，四川 雅安 6 2 5 0 1 4 ) 摘要：为了检测湖北襄阳地区规模化牛场产气荚膜梭菌(Clostridium per fringens)的分布现状，调查健康牛群中该菌的流行情况，从襄阳地区4个规模化牛 场采集样品进行产气荚膜梭菌的分离和鉴定。经过选择性培养基分离培养，结合菌落特征、革 兰氏染色镜检和生化试验鉴定，结果从6 8个新鲜粪便样品和2 4个环境样品中共分离得到产 气荚膜梭菌8株，分离率为8.7%。表明襄阳地区健康牛群中的产气荚膜梭菌与报道的国内 其他地区相比检出率较低。 关键词：牛场；产气荚膜梭菌(Clostridium perfringens)；分 布现状；分离；鉴定；襄阳地区 中图分类号：S852.61；S858.23 文献标识码：A 文章编号：0439- 8 114 ( 2 0 11) 23-4893-03 Isolation and Identification of Clostridi um perfringens from Scale Cattle Farms in Xia ngyang,Hubei ZHENG Xiao-li1,DOU Xian-ming1,HU Dao—jun2, XIAO Yong1,ZHAO Cui—rong1,NI Xue—qin3 (1. Xiangya ng Acad emy o f Agri c u l t u ra l S c i e nce i n Hubei, Xi angy ang 4 4 1057, H u b e i, C h i n a； 2. Angus Ca ttl e Fa rm o f H ubei P r o v i nc e, X i a ngyang 4 4 1 1 0 6, Hubei, China； 3. Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultura l University,Yaan 625014,Sichuan,China) A b strac t ： The C l o s tri dium p erfringens i nt h es a mples f rom 4 s c a le cattl e farms were i so l at e d and i d entif i e d to test t he distribu t io  n of C. perfring iangyang of Hube n heh se1thy cattlective isoens in scale cattle herds in X i and the epidemic situation i e herds was investlated culture, rfringens were isolatedigate. Througrains of6 8 freshstcalsampl n of C. perfring iangyang of Hube n he h se 1thy cattlective iso ens in scale cattle herds in X i and the epidemic situation i e herds was inve st lated culture, rfringens were isolated igate. Througrains of6 8 fresh st c a l s a mpl e s an d2 4 env ironme nt a l sam p l e s c on b i n i n g th e c ol ony char ac t e ri st i c s and t h e re s u l t s of m ic ro sco pic e xa m i na ti o n aft e r g r am s t a i n ing a n db iol ogic al c h ar ac t e ris t i c s . It s h o w e d th a t th ed etect io n ra te o f C. p e r f r ing e n in the h e