(4.3.5)--厨余垃圾厌氧发酵失稳调控及微生物群落分析.pdfVIP

厨余垃圾厌氧发酵失稳调控及微生物群落分析

摘要：针对厨余垃圾厌氧发酵过程中容易积累丙酸和丁酸导致反应体系酸化失稳的问题,驯化了富集耐丙酸和耐丁酸厌氧发酵菌群的接种菌泥,探究利

用其对厨余垃圾干式厌氧发酵酸化失稳体系进行调控后对甲烷产量和微生物群落的影响.酸化失稳厌氧体系中添加耐丙酸菌泥调控后,与空白对照组

相比反应体系中丙酸浓度削减6900.81mg/L,累积甲烷产量提升了259%;添加耐丁酸菌泥调控后,反应体系中丁酸浓度削减5371.56mg/L,累积甲烷产量

提高了210%.微生物多样性分析表明,利用耐丙酸和耐丁酸菌群调控后,细菌种群丰富度明显提高.细菌属水平分析表明,投加耐丙酸驯化菌群后有利于

Sporanaerobacter和Proteiniphilum等挥发性脂肪酸降解菌相对丰度增加;投加耐丁酸驯化菌群后,与乙酸型产甲烷菌有协同作用Sporanaerobacter相

对丰度有所增长.古菌属水平分析表明耐丙酸和丁酸菌调控后,氢型产甲烷菌Methanobacterium和乙酸型产甲烷菌Methanosaeta的相对丰度明显提高.

关键词：厨余垃圾；干式厌氧发酵；酸化失稳调控；微生物群落

中图分类号：X705,X703.5文献标识码：A文章编号：1000-6923(2022)04-1770-10

DOI:10.19674/j.cnki.issn1000-6923.2022.0086

Regulationofacidifieddryanaerobicdigestionofkitchenwasteandmicrobialcommunityanalysis.WANGPan,YANG

*

Xin-yu,ZHENGYi,RENLian-hai(StateEnvironmentalProtectionKeyLaboratoryofFoodChainPollutionControl,Schoolof

EcologyandEnvironment,BeijingTechnologyandBusinessUniversity,Beijing10048,China).ChinaEnvironmentalScience,

2022,42(4)：1770~1779

Abstract：Aimingattheproblemscausedbytheaccumulationofpropionicacidandbutyricacidduringanaerobicdigestion,the

sludgewithpropionicacidandbutyricacidtolerantmicroorganismweredomesticated,andtheywereaddedintotheacidified

systemsofdryanaerobicdigestionofkitchenwastetoexploretheeffectsonmethaneproductionandmicrobialcommunities.After

treatedbythesludgewithpropionicacidandbutyricacidtolerantbacteria,thepropionicacidconcentrationreducedby6900.81mg/L,

andthecumulativemethaneproductionincreasedby259%,whilethebutyricacidconcentrationreducedby5371.56mg/L,andthe

cumulativemethaneproductionincreasedby210%,respectively.Therichnessofbacteriaintheacidifiedsystemswassignificantly

improvedaftertheregulationwit