(4.3.8)--复配微生物菌群修复石油污染土壤的实验研究.pdfVIP

复配微生物菌群修复石油污染土壤的实验研究

摘要：为了修复沿海地区的石油污染场地，该文通过提取驯化土著微生物菌，筛选复配了高效石油降解菌群，在适宜降解条件

下（盐度添加比为1%、温度为25℃、pH为7），开展高效石油降解菌群修复石油污染土壤的生物强化实验。结果表明，以萃取油混合

菌、表面活性剂产生菌、萘降解菌、蒽降解菌、芘降解菌按10∶1∶1∶1∶1的比例复配的高效降解菌群在相同的时间内降解效率最高，在

第24天对2000mg/L石油烃提取液的降解率达到82.0%；添加锯末（60mg/g）、营养盐（C∶N∶P为100∶10∶1）和每天振荡的条件为最佳强

化组合方式，菌群的接种量为2%，在第48天降解率就达到了88.0%，石油污染物从初始浓度50.0mg/g降解为6.0mg/g。

关键词：石油污染土壤；石油烃降解菌；降解率；生物刺激；生物强化

中图分类号：X172文献标志码：Adoi：10.19672/ki.1003-6504.2020.S2.011文章编号：1003-6504(2020)S2-0074-05

ExperimentalStudyontheCombinationofMicrobialDegradationBacteria

toRemediateOil-contaminatedSoil

TIANXizhao，YUANZiting，GONGZhiqiang，SHANQiang，*

LIHongchao，QIShaoqian，WANGYan*

（HebeiGeo-environmentMonitoring,HebeiKeyLaboratoryofGeologicalResourcesandEnvironmentMonitoringandProtection,

Shijiazhuang050021,China）

Abstract：Inordertoremediatetheoilcontaminatedsoilincoastalareas,indigenousmicroorganismswereextractedanddo⁃

mesticated，andhighlyefficientpetroleum-degradingbacteriawerescreenedandcompounded.Undersuitabledegradation

conditions(salinityadditionratioof1%,temperatureof25℃,pHof7),bio-enhancementexperimentswerecarriedouttoeffi⁃

cientlyrepairtheoil-contaminatedsoilbydegradingbacteria.Theresultsshowedthat,inthesametime,thehighlyefficient

degradingbacteriacompoundedintheratioof10∶1∶1∶1∶1withextractoilmixedbacteria,surfactant-producingbacteria,

naphthalene-degradingbacteria,anthracene-degradingbacteria,andpyrene-degradingbacteriahadthehighestdegradation

efficiency.Remedying24daysundertheseconditions,theoildegradationrateofabout2000mg/Lreached82.0%.Thebest

combinationwasaddsawdust(60mg/g),nutrients(C∶N∶Pis100∶10∶1)anddailyshaking.Theinoculationamountofthe

florais2%,andthedegradationratereached88.0%onthe48thday,thepetroleump