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用流式细胞仪分析脂肪细胞聪明出于勤奋，天才在于积累

AnalysisandIsolationofAdipocytes

byFlowCytometry

ang

2016-0405

MajkaSM,MillerHL,HelmKM,etal.AnalysisandIsolationofAdipocytesbyFlowCytometry

Methodsinenzymology2014537:281296.d01:10.101689780100015×

CONTENTS

Background

Aimoftheresearch

Difficult

Solution

Backound

Whatareadipocytes(fatcells)?

Majorcellsintheadiposetissu

Thestructureofadiposetissuedeeptotheskin

LMX340

Backound

Twokindsofadipocytesinhuman

Lipiddroplet

hitefatcell

BrownFatCell

Backound

Prospectsofadipocytebiology

Lipidstore

Leptin

Inhibiteating

Increasemetabolism

Backound

Prospectsofadipocytebiology

IAdiponectin

MCP-l

Visfatin

ipidstore

Adipokines

Involvedinenergymetabolism

Backound

Prospectsofadipocytebiology

Specificallyfromadipose

tissue

IAdiponectin

MCP-l

Visfatin

Bloodvesse

ipidstore

Energymetabolism

Backoun

Prospectsofadipocytebiology

Adipokinesplayansignificantroleinbiologicalfunction

Proteinsinvolvedin

(e.g,plasminogenactivato

Regulatorsofblood

nhibitor-1,tissuefactor)

Hormone-likeproteins

(e.g,leptinand

ADIPOKINES

Acute-phase

Classicalcytokines

(e.g,C-rea

eptide)

Promotersofangiogenesis

(e.g,vascularendothelia

growthfactor)

Backound

Principlediagram

Detector

Flowcytometry(FCM)

Backound

Volume

Detector

Morphological

complexityofcells,

TotalDNA/RNA

content

Apoptosis

Cellviability.

10

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