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Received:29June2020Revised:7November2020Accepted:16November2020
DOI:10.1002/yea.3539
RESEARCHARTICLE
Constructionandcharacterizationofazinc-induciblegene
expressionvectorinfissionyeast
ShinyaTakahata1|TakahiroAsanuma2|MiyukiMori2|YotaMurakami1
1DepartmentofChemistry,FacultyofScience,
HokkaidoUniversity,Sapporo,JapanAbstract
2GraduateSchoolofChemicalScienceandGeneexpressionvectorsareusefulandimportanttoolsthatarecommonlyusedina
Engineering,HokkaidoUniversity,Sapporo,
varietyofexperiments,includingexpressionofforeigngenes,functionalanalysisof
Japan
genesofinterestandcomplementationexperiments.Inthisstudy,ahybridpromoter,
Correspondence+
combiningtheadh1upstreamactivatingsequence(UAS)offissionyeastandthe
ShinyaTakahata,BioorganicChemistry
Laboratory,DepartmentofChemistry,FacultyGAL10corepromoterofbuddingyeast,wasconstructedtoenablehighlevelexpres-
ofScience,HokkaidoUniversity,N10W8
siondependingonthepresenceofzincinculturemediumforfissionyeast.When
Kita-Ku,SapporoCity,Hokkaido060-0810,
Japan.thehybridpromoterwasclonedonthemulticopyplasmid,itwasfullyinducedand
Email:shinya.takahata@sci.hokudai.ac.jp
repressedwithin10hinthepresenceandabsenceofzinc,respectively.Thekinetics
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