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liangetal.JournalofExperimentalClinicalCancerResearch2012,31:64
/content/31/1/64
RESEARCHOpenAccess
Constructionofarecombinanteukaryotic
expressionvectorcontainingPHD3geneandits
expressioninHepG2cells
1*†2†1113
Qi-LianLiang,Zhou-YuLi,YuanZhou,Qiu-LongLiu,Wen-TingOuandZhi-GangHuang
Abstract
Prolylhydroxylasedomain3(PHD3)isahypoxiainduciblefactor-α(HIFα)regulator;itdegradesHIFαinthe
presenceofoxygen.Recently,therehavebeenanincreasingnumberofstudiesabouttheroleofPHD3in
proliferationandapoptosisofcancercells.However,mostoftheevidencefortheroleofPHD3isobservational,
andlittleisknownofthemolecularmechanism.Inourcurrentstudy,weconstructedarecombinanteukaryotic
expressionvectorcontainingthePHD3geneanddetecteditsbiologicalactivityinhumanhepatomacellline
(HepG2cells).WesuccessfullyconstructedarecombinantpcDNA3.1(+)-PHD3plasmid;theresultsshowedthat
PHD3overexpressioncouldinhibittheproliferationofHepG2cellsandinduceapoptosisbyactivatingcaspase-3
activity.OurstudyhasprovidedpreliminarymaterialsanddataforfurtherinvestigationoftheeffectofPHD3on
HepG2cells.
Keywords:Prolylhydroxylasedomain3(PHD3),Hepatocellularcancer(HCC),Hypoxiainduciblefactor(HIF),
Caspase-3
Introductionappearstoinduceapoptosisincancercells[11-13].How-
Therearethreeprolylhydroxylasedomainproteinsever,mostofthesestudiesareobservational,andknow-
(PHDs),PHD1,PHD2andPHD3,thatarethekeyre-ledgeofPHD3’smolecularmec
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