HPLC-ELSD法测定三乙醇胺单、双、三酯季铵盐的含量.docx

HPLC-ELSD法测定三乙醇胺单、双、三酯季铵盐的含量

杨肖;孙清;孙玲;石海英

【摘要】Amethodforthedeterminationoftriethanolaminemonoester,diesterandtriesterammoniumsaltbyNP–HPLC–ELSDwasestablished.Mobilephasewashexane–methanol–tetrahydrofuran(85∶10∶5,pHwasadjustedto3.50byadding0.05mLtrifluoroaceticacid).ChromatographiccolumnwasHP–Aminopositivephasecolumn,thecolumntemperaturewas27℃,theflowratewas1.0mL/min,evaporativelight-scatteringdetectordrifttubetemperaturewas80℃,andcarriergasflowratewas2.6L/min.Naturallogarithmofmassconcentrationoftriethanolaminemonoester,di