针刺治疗慢性心肌缺血小型猪模型系列方法的可行性探讨 .pdfVIP

针刺治疗慢性心肌缺血小型猪模型系列方法的可行性探讨

张金铃;孟宏;田毅;荣培晶;方继良;王瑜;石力;贾术永;何勋;雷洪涛

【摘要】ObjectiveToexplorethefeasibilityofneedle-embeddingtherapy

inthetreatmentofchronicmyocardialischemiausingaminiaturepig

modelestablishedbyplacementofanAmeroidconstrictorattheleft

anteriordescendingbranch(LAD)ofcoronaryarteryinBamaminiature

pigsduringsurgery.MethodsTheminiaturepigmodelofchronic

myocardialischemiawasestablishedbyplacementofanAmeroid

constrictorattheleftanteriordescendingbranchoftheleftcoronary

arteryinBamaminiaturepigs.Thepigmodelswererandomlydividedinto

thetreatmentgroup(theNeiguangroup)andthecontrolgroup(the

Zusanligroup),andweretreatedwithneedle-embedding

electroacupunctureattheNeiguan(PC6)andZusanli(ST36)acupoints,

respectively.Myocardialsamplesweretakenat6weeksaftersurgeryfor

lightandelectronmicroscopicexaminations.ResultsGrosspathology

showedthatischemicareainthemyocardiumappearedinboth

experimentalgroups.TheischemicareaintheZusanligroupwaslarger

thanthatoftheNeiguangroup.Histopathologyshowedthatthe

acupuncturetreatmentattheNeiguanacupointreducedtheischemic

injuryinthepigmyocardialtissues.Ultrastructuralobservationofthe

myocardiumshowedmitochondrialvacuolizationincardiomyocytesand

myocardialfibrosisinbothgroups.ConclusionsAcupuncturetherapyat

theNeiguanacupointofpericardialchannelmayexertprotectiveeffecton

themyocardialischemiabyreducingtheischemia-injuryof

cardiomyocytes,butcannotinhibitthealreadyexistedischemia-induced

cardiomyocyticinjuries.Ourfindingssuggestthattheestablishmentof

miniaturepigmodelofchronicmyocardialischemiabysurgicallyplacing

anAmeroidconstrictorontheleftanteriordescendingbranchofleft

coronaryarteryandtheneedle-embeddinginacupointsisfeasibleforthe

treatmentofchronicmyocardialischemiainthispigmod