气相色谱质谱原理.ppt

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2024-12-19 发布于广东
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气相色谱质谱原理.ppt

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**IwillexplaintheprincipleofEI.EIappliestheelectrongeneratedfromthefilamenttoagas-likesample.Amoleculeisdestroyedbytheenergyofthiselectron.First,anelectronispulloutfromamolecule,thenthisionturnsintomoleculeionwhichshowsamolecularweight.Thismoleculeionisveryimportant.Furthermore,itbreaks,andfragmentionsarise.Wegetthestructureinformationfromthisfragmentions.QualitativeandQuantitativeanalysisareperformedusingmoleculeionandfragmention.However,whenelectronicenergyislargerthantheenergywhichamoleculehas,amoleculeionpeakdoesnotappear,thenitbecomesfragmentionsuddenly,andonly.Inthiscase,molecularweightdeterminationisdifficult.Insuchacase,weusetheCImethod.IshowthemassspectrumofstearicacidmethylesterasanexampleofEI.M/z298isaverysmallpeak,butitisveryimportantion,becauseitisamoleculeionpeak.Andthefragmentionsofm/z74and87showthestructureoffattyacidmethylester.**Title: “UltimateProductivityintheDrugTestingLaboratory”Author: NickBukowski FinniganMassLab CreweRoad Wythenshawe Manchester M239BE UKTel: +44-61-946-1060Fax: +4-161-998-988Email: bukowskin@finnigan.co.ukCompuServe: 75664,3542FinniganMassLab-adivisionofThermoQuestCorporation**Next,IwillexplainCI.Thereare2methodsinCI.OneisPCIforpositiveion,anotherisNCIfornegativeion.ForPCIweintroducereagentgasintotheionsource.ForreagentgasweuseMethane,isobutane,orammonia.Anelectronisappliedtothisreagentgas.Reagentgasservesasion.Thisionandasamplemoleculecauseanionmoleculereaction,andasamplemoleculeservesasion.Inthiscase,bythereasonwhyenergyissmall,soalmostmoleculeionisdetected.Anditbecomestheionwhichtheprotongenerallyaddedtothemolecule,soitbecomestheionaddedtothemolecularweightonetime.Moreover,mostfragmentation