基于UPLC-QQQ-MS和PLS-DA分析2种寄主鲜品肉苁蓉苯乙醇苷成分差异.docxVIP

基于UPLC-QQQ-MS和PLS-DA分析2种寄主鲜品肉苁蓉苯乙醇苷成分差异

吕霞1，郭晔红1*，高培文1，张文瑶1，王怡维1，贾存勤2(1.甘肃农业大学农学院，干旱生境作物学国家重点实验室，兰州730070；2.甘肃汇勤生物科技有限公司，甘肃白银730600)

摘要：目的比较评价鲜品四翅滨藜-肉苁蓉与梭梭-肉苁蓉汁和渣中苯乙醇苷成分含量的差异。方法通过超高效液相色谱-三重四极杆串联质谱联用法(ultraperformanceliquidchromatography-triplequadrupoletandemmassspectrometry，UPLC-QQQ-MS)，对2种寄主鲜品肉苁蓉汁和渣中8种苯乙醇苷成分含量进行比较，并结合偏最小二乘判别分析(partialleast-squaresdiscriminant-analysis，PLS-DA)，鉴定并评价2种寄主鲜品肉苁蓉的苯乙醇苷成分差异。结果四翅滨藜-肉苁蓉汁和渣中苯乙醇苷含量均高于梭梭-肉苁蓉；经过杀酶5min的四翅滨藜-肉苁蓉和梭梭-肉苁蓉渣，再经80℃干燥的肉苁蓉苯乙醇苷含量较未杀酶直接干燥的肉苁蓉分别高出131.5108%和93.2983%。结论以肉苁蓉的主要成分苯乙醇苷为评判指标，可快速、高效地评估鲜品四翅滨藜-肉苁蓉与梭梭-肉苁蓉汁与渣中活性成分的差异。且鲜品四翅滨藜-肉苁蓉渣的苯乙醇苷含量更高，杀酶5min可有效保留鲜品肉苁蓉苯乙醇苷的含量。

关键词：超高效液相色谱-三重四极杆串联质谱联用法；四翅滨藜；梭梭；苯乙醇苷；鲜品肉苁蓉

中图分类号：R284.2文献标志码：B文章编号：1007-7693(2025)09-1463-08

DOI:10.13748/ki.issn1007-7693引用本文：吕霞,郭晔红,高培文,等.基于UPLC-QQQ-MS和PLS-DA分析2种寄主鲜品肉苁蓉苯乙醇苷成分差异[J].中国

现代应用药学,2025,42(9):1463-1470.

DifferenceofPhenylethanolGlycosidesinFreshCistanchesHerbafrom2HostsBasedonUPLC-QQQ-MSandPLS-DAAnalysis

LYUXia1,GUOYehong1*,GAOPeiwen1,ZHANGWenyao1,WANGYiwei1,JIACunqin2(1.AgronomyCollege,GansuAgriculturalUniversity,NationalKeyLaboratoryofAridlandCropScience,Lanzhou730070,China;2.GansuHuiqinBiologicalTechnologyCo.,Ltd.,Baiyin730600,China)

ABSTRACT:OBJECTIVETocomparativelyevaluatethedifferencesofphenylethanolglycosidescontentinthejuiceandresidueoffreshCistanchesHerbawiththehostsofAtriplexcanescensandHaloxylonammodendron.METHODSThecontentsofeightphenylethanoidglycosidesinthejuiceandresidueofsamplesderivedfromtwodifferenthostplantswerecomparedusingultraperformanceliquidchromatography-triplequadrupoletandemmassspectrometry(UPLC-QQQ-MS).Furthermore,partialleastsquaresdiscriminant-analysis(PLS-DA)wasemployedtoidentifyandevaluatethedifferencesinphenylethanoidglycosideprofilesbetwee