响应面法对金银花叶总黄酮与绿原酸同步萃取工艺的优化.pdfVIP

山东化工

·4·SHANDONGCHEMICALINDUSTRY2024年第53卷

响应面法对金银花叶总黄酮与绿原酸同步萃取工艺的优化

李华生，周振华，骆航，龙鸣，陈瑛

（永州职业技术学院，湖南永州425100)

摘要：采用响应面法，对加压辅助溶剂同步萃取金银花叶总黄酮和绿原酸的工艺进行了优化。在单因素试验的基础上，综合评价总黄酮

和绿原酸的得率“OD值”作为因变量，通过响应面法优化了液料比、萃取压力、溶剂体积分数、萃取温度和萃取时间。结果表明，优化后

的加压辅助溶剂同步萃取工艺参数为：液料比26.5mL/g，萃取压力3.1MPa,溶剂体积分数60.5%，萃取温度90.3℃，萃取时间32min。

该工艺条件下，从金银花叶中萃取总黄酮得率为205.3mg/g，绿原酸得率为24.5mg/g，0D值与理论预测值的相对误差为1.0%。

关键词：响应面分析；金银花叶；总黄酮；绿原酸；加压萃取工艺

中图分类号：TQ461;TS05;TS202.3文献标志码：A文章编号：1008-021X(2024)12-0004-05

OptimizationoftheSimultaneousExtractionProcessofTotalFlavonoidsand

ChlorogenicAcidfromHoneysuckleLeavesbyResponseSurfaceMethod

LiHuasheng,ZhouZhenhua,LuoHang,LongMing,ChenYing

(YongzhouVocationalandTechnicalCollege,Yongzhou425100,China)

Abstract:Theprocessofsimultaneousextractionoftotalflavonoidsandchlorogenicacidfromhoneysuckleleavesbypressurized

auxiliarysolventwasoptimizedbyusingtheresponsesurfacemethod.Onthebasisofsinglefactorexperiment,theyieldoftotal

flavonoidsandchlorogenicacidwascomprehensivelyevaluatedasthedependentvariable,andtheliquid-materialratio,extraction

pressure,solventvolumefraction,extractiontemperatureandextractiontimewereoptimizedbyresponsesurfacemethod.The

resultsshowedthattheoptimizedprocessparametersofpressurizedauxiliarysolventsynchronousextractionwereasfollows:liquid

-materialratio26.5mL/g,extractionpressure3.1MPa,solventvolumefraction60.5%,extractiontemperature90.3℃,and

extractiontime32min.Undertheconditionsofthisprocess,theyieldoftotalflavonoidsextractedfromhoneysuckleleaveswas

205.3mg/g,theyieldofchlorogenicac