《医学检验技术》课件—— Cerebrospinal Fluid Analysis 脑脊液检查.pptxVIP

;;Procedure

Sterilelumbar[?l?mb?]puncture(L3-L4/L4-L5),Collect3tubes:

①Microbiology无菌微生物管

②Biochemistry生化管

③Cytology细胞学管

TubesequenceoftesttubesiscriticalwhencollectingCSF.试管顺序至关重要

Tube1forcultureavoidsskincontamination.第1管做培养避免皮肤污染

Thesecondisusedforbiochemicalexaminationandthethirdforcytology.

Immediatetransportwithin1hourpreventscelllysisandglucosedecreases[d??kri?s?z].一小时内立即送检防止细胞溶解和葡萄糖降解;MacroscopicExamination感官检查;BiochemistryTests;1.GramStain革兰染色

FindingGram-negativediplococci[?d?pl???k?ksa?]suggests

Neisseria[na??s??r??]meningitis[?men?n?d?a?t?s].

发现革兰阴性双球菌提示为脑膜炎奈瑟菌;2.India[??ndi?]inkstain印度墨汁染色

Indiainkhighlightsfungalcapsulesthroughnegativestaining.

通过负染色突出真菌荚膜

Theinksdarkbackgroundcontrastswiththeclearhalo[?he?l??]aroundcryptococcus[kr?p?t?k?k?s].

Thisisdiagnosticforcryptococcalmeningitis。

墨汁的黑色背景与隐球菌周围的透明环形成鲜明对比

可诊断隐球菌脑膜炎;3.AcidFaststain抗酸染色

Theacidfaststaindetectsacid-fastbacilli[b??s?la?].抗酸染色检测抗酸杆菌

Mycobacterialiketuberculosis[tju??b??kju?l??s?s]blacili[b??s?la?]resistdecolorization[di??k?l?ra??ze???n]byacid-alcohol,

appearingasredrodsagainstabluebackground.

如结核分枝杆菌能抵抗酸性酒精的脱色，在蓝色背景下呈红色棒状;4.CytologyAnalysis细胞学分析

Celldifferentialisdiagnostic:细胞分类具诊断意义

Neutrophils70%suggestsbacterialmeningitis;

中性粒细胞70%提示细菌性脑膜炎

Lymphocytosisindicatesviralormycobacteriumtuberculosisinfection.

淋巴细胞增多指向病毒或结核杆菌感染;Rememberourtriad[?tra??d]核心三点

Propercollectionpreventserrors[?er?z],规范采集防差错

rapidprocessingpreservesaccuracy,快速检测保准确

Integrated[??nt?ɡre?t?d]interpretationprotectslives.综合判读护生命

CSFanalysisisaraceagainsttime!脑脊液检查是与时间的赛跑!;ThankYou!