文献汇报(高速逆流色谱和制备高效液相色谱法-分离贯叶金丝桃中的儿茶素和黄酮类化合物).pptVIP

SeparationofepigallocatechinandflavonoidsfromHypericumperforatumL.byhigh-speedcounter-currentchromatographyandfollowedbypreparativeliquidchromatography

高速逆流色谱和制备高效液相色谱法，

分离贯叶金丝桃中的儿茶素和黄酮类化合物AcceptbyJournalofChromatographyAAuther：WeiY,XieQ,DongW,ItoY.May8IF:4.1695-yearIF:4.298

Introduction2.Experimental3.Resultsanddiscussioncontent4.Conclusions

HypericumperforatumL.贯叶金丝桃IntroductionNaphthodianthrones:hypericin金丝桃素Phloroglucinols:hyperforin贯叶金丝桃素Flavonoids黄酮类:quercetin槲皮素,quercitrin槲皮苷Phenolicacids:chlorogenicacid绿原酸Epigallocatechin表焙儿茶素etc.

IntroductionHigh-SpeedCountercurrentChromatography(HSCCC)高速逆流色谱技术asupport-freeliquid–liquidpartitionmethod液-液色谱分离技术eliminatesirreversibleadsorptionofsampleontothesolidsupport无不可逆吸附

HSCCCLowerphasePeakfractionExperimentalCrudeextractPrep-LCHPLCHSCCC:Preparationoftwo-phasesolvent

ExperimentalSeparationprocedureHSCCC：Thelowerphase：mobilephaseflowrate:2.0ml/min；revolutionspeed:800rpmTheupperphase：stationaryphaseUVdetectorat254nm

Preparationoftwo-phasesolventandsamplesolutionforHSCCCHSCCC中两相溶剂和样品的制备n-hexane-ethylacetate-methanol-water正己烷-乙酸乙酯-甲醇-水(1:1:1:1,v/v)solventsystemethylacetate-methanol-water乙酸乙酯-甲醇-水(5:1:5or10:1:10or50:1:50,v/v)samplesolutiondissolvingthecrudeextractinthelowerphaseExperimental

ExperimentalHPLCanalysesandidentificationofHSCCCpeakfractionsYMCPackODS-A,150×4.6mmI.D.ODScolumn;eluentA:MeOH—eluentB:0.08%H3PO4inwatergradientelutionTime(min)B(%)0955702555305035504595flowrate:0.8ml/minsamplequantity:10μlFlavonoids黄酮类UVdetectionwavelength：254nm

Fig.2ethylacetate-methanol-water50:1:50(v/v)(a)；10:1:10(v/v)(b)；5:1:5(v/v)(c),Peak2(inFig.2a,2b,2c):mixtureofhyperoside,isoquercitrinandmiquelianin.Peak3:mixtureofepigallocatechinandavicularin.Peak4:quercitrin

Fig.4

Conclusions900mgcrudeextract7.8mgquercetrin槲皮素(apurityofove