石斛毛状根诱导及培养条件优化研究.pdfVIP

2,等三七试管苗繁殖技术的研究华南农业大

参考文献学学报,1992,13(3)69

3,等三七胚培养中的胚胎发生植物学通报,

1许鸿源,等灵发素(LFS)对三七愈伤组织发生和增殖

1989,6(4)236

的影响中药材,2004,27(1)1

(2004-03-08收稿)

EffectsofLingfasuonEmbryoidandntlet

FormationofPanaxnotoginsenginVitro

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XuHongyuan,MengAidong,LiChunxia,DengXiqing,ZhouQiwei,YangMeichun

(1AgriculturalCollege,GuangxiUniversity,Nanning530005;2MedicinalntGardenofGuangxi,Nanning530001)

OntheMSaddedtherightamountof2,4DandLFS(Angustmycin)andculturedunderdarkcondition,the

callusfromstemsegmentsofPanaxnotoginsengcouldinducealotofembryoidsIn2~3months,theratioofembryoidformation

reachedabout90%ThentransntedonMS+2,4D15mg/L+LFS2mg/Landculturedunderlight2000lx,near30%embry

oidscouldgrowanddevelopasrobustntlets

KeywordsLingfasu(LFS,Angustmycin);Panaxnotoginseng;Embryoid;ntlet

石斛毛状根诱导及培养条件的优化

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