暨南大学环境污染与健康-第4章 有机定量分析中的质量保证与质量控制.pptVIP

Selectionoftargetanalytes(目标分析物)Selectionofsurrogatestandards(回收率指示物)Selectionofinternalstandards(内标)SelectionofanextractionmethodSelectionofadetectionmethod(typeofinstrument)EvaluationofthemethodperformanceMethodDevelopmentforQuantitativeAnalysisGaschromatography(GC)Gaschromatography/massspectrometry(GC/MS)Time-of-flightmassspectrometry(TOF-MS)Liquidchromatography/massspectrometry(LC/MS)Acceleratormassspectrometry(AMS)分析仪器的种类Selectionoftargetanalytes(目标分析物)Selectionofsurrogatestandards(回收率指示物)Selectionofinternalstandards(内标)SelectionofanextractionmethodSelectionofadetectionmethod(typeofinstrument)EvaluationofthemethodperformanceMethodDevelopmentforQuantitativeAnalysisSelectionofConcentrationLevels：Fivetosixcalibrationsolutionscontainingtargetanalytesataseriesofincrementalconcentrations,startingfromabout5-10timesIDL,arepreparedwithinternalstandards.Relativeresponsefactors(RRFs)areobtainedateachconcentrationlevel,withstandarddeviations(RSDs)lessthan15-20%DeterminationofInstrumentDetectionLimit(IDL):Analyze7samplesspikedwithtargetanalytesatlevels~5timestheS/N,andcalculatethestandarddeviations(S)IDL=3.14*SDeterminationofCalibrationRangeAnalysisof7matrixspikingsamplescontainingtargetanalytesatlevels~5timesIDLsCalculationofstandarddeviations(S)MDL=3.14*SDeterminationofMethodDetectionLimitForeachsetof10to20fieldsamples,thefollowingnon-fieldsamplesneedtobeanalyzed:A.Methodblank(方法空白)B.Spikedblank(加标空白)C.Matrixspikedsample(基质加标)D.Matrixspikedduplicate(基质加标平行样)E.Sampleduplicate(样品平行样)F.Laboratorycontrolstandard/Standardreferencematerial(标准参考物质),e.g.,NISTSRM1941QCMeasuresinAnalysisofFieldSamplesORRecoveriesofthetargetanalytesinspikedsamplesmustbemostlywithin80-120%Thelaboratorymustestablish