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- 2026-02-27 发布于江西
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落叶果树2025,57(6):22-27
·试验研究
DeciduousFruits
DOI:10.13855/j.cnki.lygs.2025.06.004
云南冷�山地72份苹果种质资源遗传多样性分
析及分子身份证的构建
赵娜斐1,刘琳1,马钧²,黄文静²,刘丹丹1*
(1.云南大学,云南昆明650091;2.云南省农业科学院园艺作物研究所,云南昆明650504)
摘要:中国苹果种质资源丰富,包括大量的野生种、半野生种及栽培品种,在生产中普遍存在同物异
名及同名异物现象,不便于种质资源的保存和利用和产业的发展。对取自云南昭通的72份苹果品种(系)
进行了遗传多样性分析,基于指纹图谱的基因型构建了分子身份证,并构建了能被快速准确识别的分子二维
码,为鉴定苹果种质资源提供了理论依据。
关键词:苹果;种质资源;SSR-PCR;遗传多样性;分子身份证
中图分类号:S661.1文献标志码:A文章编号:1002-2910(2025)06-0022-06
Geneticdiversityanalysisandmolecularidentificationcardconstructionof72apple
accessionsinthecoolmountainousareasofYunnan
ZHAONafei1,LIULin1,MAJun2,HUANGWenjing2,LIUDandan1
(1.YunnanUniversity,Kunming,Yunnan650091,China;2.InstituteofHorticulturalCrops,YunnanAcademyof
AgriculturalSciences,Kunming,Yunnan650504,China)
Abstract:Chinaboastsabundantapplegermplasmresources,encompassingalargenumberof
wildspecies,semi-wildspecies,andcultivatedvarieties.However,thewidespreadphenomenaof
synonymy(differentnamesforthesamegermplasm)andhomonymy(thesamenamefordifferent
germplasms)inproductionhavehinderedtheconservationandutilizationofgermplasmresources
aswellasthedevelopmentoftheappleindustry.Inthisstudy,geneticdiversityanalysiswas
conductedon72applevarieties(lines)collectedfromZhaotong,YunnanProvince.Molecular
identificationcardswereconstructedbasedonthegenotypesderivedfromfingerprinting,and
molecularQRcodesenablingrapidandaccurateidentificationwerealsodeveloped.Thisresearch
providesatheoreticalbasisfortheidentific
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